| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLDN |
| Uniprot No | Q9UL45 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MSVPGPSSPD GALTRPPYCL EAGEPTPGLS DTSPDEGLIE DLTIEDKAVE QLAEGLLSHY LPDLQRSKQA LQELTQNQVV LLDTLEQEIS KFKECHSMLD INALFAEAKH YHAKLVNIRK EMLMLHEKTS KLKKRALKLQ QKRQKEELER EQQREKEFER EKQLTARPAK RM |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLDN(假设指Pallidin，BLOC-1复合体成员)重组蛋白研究的模拟参考文献示例，供参考：
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1. **文献名称**：*Recombinant Expression and Functional Analysis of Pallidin in BLOC-1 Complex Dynamics*
**作者**：Chen L, et al.
**摘要**：报道在大肠杆菌中成功表达并纯化重组Pallidin蛋白，证实其与BLOC-1复合体的其他组分结合，调控溶酶体相关细胞器的膜运输过程，为Hermansky-Pudlak综合征机制提供新见解。
2. **文献名称**：*Structural Insights into Pallidin via Recombinant Protein Crystallography*
**作者**：Martinez S, et al.
**摘要**：通过重组表达人源Pallidin蛋白并进行晶体结构解析，揭示了其N端结构域在BLOC-1复合体组装中的关键作用，提出突变位点对疾病表型的影响机制。
3. **文献名称**：*High-Yield Production of Functional PLDN in Insect Cells for Interaction Studies*
**作者**：Wang Y, et al.
**摘要**：利用杆状病毒-昆虫细胞系统高效表达重组PLDN蛋白，结合pull-down实验鉴定其与AP-3适配体的相互作用，阐明其在囊泡分选通路中的功能。
4. **文献名称**：*PLDN Recombinant Protein Rescue in Cellular Models of Lysosomal Dysfunction*
**作者**：Gomez F, et al.
**摘要**：在PLDN缺陷细胞中导入重组PLDN蛋白，恢复溶酶体运输功能，证明其在黑素体形成中的必要性，为基因治疗提供实验依据。
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**说明**：
- 上述文献为模拟示例，实际研究中请通过PubMed、Google Scholar等平台以关键词“Pallidin recombinant protein”“BLOC-1 complex”或“PLDN gene”检索最新文献。
- 若PLDN指其他蛋白(如特定病毒或细菌蛋白)，建议结合具体研究领域调整检索策略。
PLDN (Pallidin), a protein encoded by the *PLDN* gene in humans, is a critical component of the Biogenesis of Lysosome-Related Organelles Complex-1 (BLOC-1). This multi-subunit complex plays a vital role in the biogenesis and trafficking of lysosome-related organelles (LROs), such as melanosomes, platelet dense granules, and synaptic vesicles. Pallidin, along with other BLOC-1 subunits (e.g., dysbindin, muted), facilitates membrane protein sorting and vesicular transport, particularly in specialized cell types like melanocytes, neurons, and hematopoietic cells.
First identified through studies of Hermansky-Pudlak syndrome (HPS), a genetic disorder characterized by albinism, bleeding disorders, and lysosomal defects, PLDN gained attention as mutations in BLOC-1 components were linked to HPS subtypes. Pallidin-deficient models exhibit disrupted LRO formation, impaired cargo trafficking (e.g., tyrosinase in melanocytes), and neurological abnormalities, underscoring its role in cellular homeostasis. Structurally, Pallidin contains conserved domains for protein-protein interactions, enabling its integration into the BLOC-1 complex and coordination with other trafficking machinery like AP-3 adaptors.
Beyond HPS, PLDN has been implicated in neurodevelopmental disorders, with altered expression observed in schizophrenia and autism models. Its involvement in synaptic vesicle recycling suggests broader neurological relevance. Recombinant PLDN protein is widely used to study BLOC-1 assembly, LRO biogenesis mechanisms, and pathological pathways. Produced via bacterial or mammalian expression systems, it serves as a tool for biochemical assays, antibody development, and drug screening targeting trafficking-related diseases. Ongoing research explores its potential as a biomarker or therapeutic target for lysosomal and neuropsychiatric disorders.
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