| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human TRPT1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于TRPT1抗体的3篇文献示例(注：TRPT1研究相对较少，以下为模拟示例，实际文献需通过数据库验证)：
1. **文献名称**: "Characterization of TRPT1 Antibody for Detection of tRNA Modification Enzymes in Human Cells"
**作者**: Smith A, et al.
**摘要**: 本研究开发了一种针对人源TRPT1蛋白的多克隆抗体，验证了其在Western blot和免疫荧光中的特异性，证实TRPT1在细胞核仁中的定位，并探讨其与tRNA 2'-磷酸转移酶活性的关联。
2. **文献名称**: "TRPT1 Knockdown Impairs Cell Proliferation and Enhances Chemosensitivity via Antibody-Based Functional Analysis"
**作者**: Chen L, et al.
**摘要**: 通过TRPT1抗体进行功能研究，发现TRPT1在癌细胞系中高表达，敲低后显著抑制细胞增殖并增强化疗药物敏感性，提示其作为肿瘤治疗靶点的潜力。
3. **文献名称**: "A Novel Monoclonal Antibody Against TRPT1 Reveals Its Role in Mitochondrial RNA Processing"
**作者**: Yamamoto K, et al.
**摘要**: 报道一种高特异性抗TRPT1单克隆抗体，用于免疫共沉淀实验，揭示TRPT1与线粒体RNA加工复合物的相互作用，为研究其在线粒体功能中的作用提供工具。
建议通过PubMed或Google Scholar以“TRPT1 antibody”为关键词检索最新文献，或查阅抗体生产商(如Abcam、Santa Cruz)的技术文档获取引用信息。
**Background of TRPT1 Antibody**
TRPT1 (tRNA 2'-O-ribosylphosphate transferase 1), also known as PTH2. is an evolutionarily conserved enzyme involved in post-transcriptional modification of transfer RNA (tRNA). It catalyzes the 2'-O-ribosylation of specific tRNA nucleotides, a critical step for maintaining tRNA stability, structural integrity, and translational fidelity. Dysregulation of TRPT1 has been linked to cellular stress responses, neurological disorders, and cancer progression, highlighting its role in fundamental biological processes.
TRPT1 antibodies are immunological tools designed to detect and quantify TRPT1 protein expression in research applications. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study TRPT1's localization, expression patterns, and interactions in various tissues and disease models. Recent studies have employed TRPT1 antibodies to explore its involvement in DNA repair mechanisms, oxidative stress adaptation, and tumorigenesis, particularly in cancers where tRNA modification pathways are aberrant.
Commercial TRPT1 antibodies are typically validated for specificity and sensitivity, often targeting conserved epitopes across species. Researchers utilize these antibodies to investigate TRPT1's functional roles in development, aging, and disease, as well as its potential as a therapeutic target or biomarker. Ongoing research aims to clarify its regulatory mechanisms and clinical relevance in human pathologies.
×
