| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLR2J2 |
| Uniprot No | Q9GZM3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-115aa |
| 氨基酸序列 | MNAPPAFESF LLFEGEKITI NKDTKVPNAC LFTINKEDHT LGNIIKSQLL KDPQVLFAGY KVPHPLEHKI IIRVQTTPDY SPQEAFTNAI TDLISELSLL EERFRTCLLP LRLLP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POLR2J2重组蛋白的模拟参考文献示例(注：以下内容为假设性文献，实际引用时需核实真实来源)：
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1. **文献名称**: "Structural and Functional Analysis of Recombinant POLR2J2 in RNA Polymerase II Assembly"
**作者**: Chen L, et al.
**摘要**: 本研究通过大肠杆菌表达系统成功制备了重组POLR2J2蛋白，结合冷冻电镜技术解析了其在RNA聚合酶II复合体中的结构，揭示了POLR2J2对转录起始的关键调控作用。
2. **文献名称**: "Recombinant POLR2J2 Enhances Transcriptional Elongation in Cancer Cells"
**作者**: Wang Y, et al.
**摘要**: 利用重组POLR2J2蛋白进行功能实验，发现其过表达促进肿瘤细胞的转录延伸过程，并与肺癌细胞增殖及化疗耐药性显著相关。
3. **文献名称**: "Optimization of POLR2J2 Recombinant Protein Production in Mammalian Cells"
**作者**: Gupta S, et al.
**摘要**: 开发了一种基于HEK293细胞的重组POLR2J2高效表达与纯化方案，为后续研究其翻译后修饰及相互作用提供了可靠工具。
4. **文献名称**: "POLR2J2 Interacts with p53: Implications for Gene Regulation"
**作者**: Martinez R, et al.
**摘要**: 通过重组POLR2J2的体外结合实验，证实其与肿瘤抑制蛋白p53直接互作，可能参与DNA损伤应答中的转录调控网络。
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**注意**：以上文献为模拟示例，实际研究中请通过PubMed、Google Scholar等平台检索真实发表的论文。若需具体文献协助，请提供更多研究背景或关键词。
The POLR2J2 recombinant protein is derived from the POLR2J2 gene, which encodes a subunit of RNA polymerase II (Pol II), the enzyme responsible for synthesizing messenger RNA (mRNA) in eukaryotic cells. As a component of the core Pol II complex, POLR2J2 (also known as RPB11B) shares homology with POLR2J (RPB11A), both contributing to the structural and functional integrity of the polymerase. These subunits are thought to play roles in transcription initiation, elongation, and interactions with regulatory factors. Unlike POLR2J, which is ubiquitously expressed, POLR2J2 exhibits tissue-specific expression patterns, suggesting specialized roles in certain cell types or developmental stages.
Recombinant POLR2J2 is typically produced in heterologous expression systems (e.g., *E. coli* or mammalian cells) for biochemical and functional studies. Its production enables researchers to investigate Pol II assembly, transcriptional mechanisms, and dysregulation in diseases like cancer or neurodegenerative disorders. Mutations in Pol II subunits, including POLR2J2. have been linked to developmental defects and cancers, highlighting its biomedical relevance. Recombinant versions are also used to study protein-protein interactions, map binding domains, or develop antibodies. Structural analyses of POLR2J2 help clarify its role in the Pol II holoenzyme and its potential as a therapeutic target. Despite its conserved function, the distinct expression and regulatory features of POLR2J2 compared to POLR2J underscore its unique contributions to transcription dynamics and cellular specificity.
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