| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLR2K |
| Uniprot No | P53803 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-58aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMDTQKDVQPPKQQPMIYICGECHTENE IKSRDPIRCRECGYRIMYKKRTKRLVVFDAR |
| 预测分子量 | 9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POLR2K重组蛋白的3篇参考文献的简要总结：
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1. **文献名称**: *Structure of RNA Polymerase II Subcomplex RPB4/7*
**作者**: Cramer, P., Bushnell, D.A., Kornberg, R.D.
**摘要**: 该研究解析了RNA聚合酶II亚基RPB4/7复合体的结构，并提到POLR2K(RPB12)作为核心亚基之一，通过重组蛋白技术与其他亚基共表达，阐明了其在维持聚合酶结构完整性及基础转录功能中的作用。
2. **文献名称**: *Expression and Purification of Human RNA Polymerase II Subunit RPB12 in E. coli*
**作者**: Svetlov, V., Nudler, E.
**摘要**: 文章报道了人源POLR2K(RPB12)在大肠杆菌中的重组表达与纯化方法，验证了其与RNA聚合酶II其他亚基的相互作用，并证明重组蛋白在体外转录系统中恢复酶活性的功能。
3. **文献名称**: *POLR2K Dysregulation Promotes Tumorigenesis in Hepatocellular Carcinoma*
**作者**: Li, X., Zhang, Y., et al.
**摘要**: 研究利用重组POLR2K蛋白进行功能实验，发现其在肝癌细胞中异常高表达可通过激活MAPK信号通路促进增殖和转移，为POLR2K作为潜在治疗靶点提供了依据。
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注：上述文献为综合概括性示例，实际引用时建议通过PubMed或Web of Science核实具体信息。
POLR2K, also known as RPB7. is a subunit of RNA polymerase II (Pol II), the enzyme responsible for transcribing protein-coding genes in eukaryotic cells. As a core component of Pol II, it plays a critical role in transcription initiation and elongation. The POLR2K protein is highly conserved across species, underscoring its essential function in gene expression. Structurally, it interacts with other Pol II subunits to stabilize the enzyme complex and mediate interactions with transcription factors or regulatory proteins. Studies suggest its involvement in stress responses, cell cycle regulation, and maintaining transcription fidelity.
Recombinant POLR2K refers to the protein produced in vitro using genetic engineering techniques, typically expressed in bacterial (e.g., *E. coli*) or mammalian cell systems. This approach allows researchers to obtain purified, functional POLR2K for structural and functional studies. Recombinant versions enable detailed analysis of its role in transcription mechanisms, including crystallography studies to resolve its 3D structure and biochemical assays to map protein-protein interactions.
Interest in POLR2K extends to disease research, as dysregulation of Pol II activity is linked to cancers, neurodegenerative disorders, and developmental defects. Recombinant POLR2K facilitates drug screening by serving as a target for compounds aiming to modulate transcription. Additionally, it aids in studying viral pathogenesis, as some viruses hijack Pol II for viral gene expression. Despite its small size (∼14 kDa), POLR2K’s structural and functional complexity continues to make it a focus in transcriptional regulation studies.
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