| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLR2J3 |
| Uniprot No | Q9H1A7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-115aa |
| 氨基酸序列 | MNAPPAFESF LLFEGEKITI NKDTKVPNAC LFTMNKEDHT LGNIIKSQLL KDPQVLFAGY KVPHPLEHKI IIRVQTTPDY SPQEAFTNAI TDLISELSLL EERFRTCLLP LRLLP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POLR2J3重组蛋白的示例参考文献(注：以下内容为模拟虚构文献，供参考格式)：
1. **"POLR2J3 promotes hepatocellular carcinoma progression via RNA polymerase II-mediated transcriptional activation"**
*作者：Wang et al. (2020)*
*摘要*：研究发现POLR2J3在肝细胞癌中高表达，通过调控RNA聚合酶II介导的致癌基因转录，促进肿瘤增殖和转移。重组蛋白实验表明其与转录延伸因子相互作用。
2. **"Structural insights into the human RNA polymerase II complex with POLR2J3 subunit"**
*作者：Zhang et al. (2018)*
*摘要*：利用冷冻电镜解析了含POLR2J3亚基的RNA聚合酶II全酶结构，揭示其参与核心酶组装和转录起始的分子机制，重组蛋白技术为结构分析提供关键支持。
3. **"POLR2J3 regulates viral RNA sensing and interferon response in innate immunity"**
*作者：Li et al. (2019)*
*摘要*：通过重组POLR2J3蛋白的功能实验，发现其与RIG-I信号通路相互作用，负调控宿主抗病毒免疫反应，提示其在免疫疾病中的潜在作用。
4. **"A novel role of POLR2J3 in maintaining pluripotency of embryonic stem cells"**
*作者：Kim et al. (2021)*
*摘要*：利用重组POLR2J3蛋白及基因编辑技术，证明其通过维持RNA聚合酶II动态磷酸化状态，影响胚胎干细胞多能性相关基因的转录延伸。
建议通过PubMed、Google Scholar等平台检索真实文献，关键词：**POLR2J3 recombinant protein, RNA polymerase II subunit, transcriptional regulation**。
The POLR2J3 recombinant protein is derived from the POLR2J3 gene, which encodes a subunit of RNA polymerase II (Pol II), the enzyme responsible for synthesizing messenger RNA in eukaryotes. Specifically, POLR2J3 is one of several paralogs (J1. J2. J3) of the POLR2J subunit, thought to have arisen through gene duplication. These paralogs may contribute to the functional diversity of Pol II complexes, potentially fine-tuning transcription in different cellular contexts or developmental stages. Recombinant POLR2J3 is produced using biotechnological methods, such as expression in bacterial or mammalian systems, often fused with tags (e.g., His-tag) for purification and detection.
Structurally, POLR2J3 retains conserved regions critical for integrating into the 10-subunit Pol II core, interacting with other subunits to stabilize the enzyme’s architecture. While its exact functional distinctions from other J paralogs remain under investigation, studies suggest paralog-specific roles in transcription regulation, stress responses, or cell-type-specific mRNA synthesis. Recombinant POLR2J3 enables in vitro studies to dissect Pol II assembly, transcriptional mechanisms, or the impact of mutations linked to diseases.
Research applications include exploring Pol II dysfunction in cancers or neurological disorders, as aberrant transcription is implicated in these conditions. Additionally, it serves as an antigen for antibody development or a tool for drug screening targeting transcriptional processes. However, challenges persist in characterizing paralog-specific contributions due to high sequence similarity among J subunits. Ongoing work aims to clarify POLR2J3’s unique interactions, regulatory roles, and potential as a therapeutic or diagnostic target in Pol II-related pathologies.
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