首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠睫状神经营养因子(CNTF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CNTF含量。CNTF即睫状神经营养因子，可促进神经元存活和分化。在它是神经营养因子家族成员。其研究机制主要围绕调节神经细胞的生长、发育和功能展开，还参与调节能量代谢等过程，在神经系统疾病等研究领域受关注。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于神经退行性疾病模型构建、神经损伤修复机制研究、药物或基因干预效果评估等科研领域，为探索CNTF在神经系统生理病理过程中的动态变化提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CntfCiliary neurotrophic factor ELISA kit; CNTF ELISA kit
缩写：CNTF
Uniprot No.：P20294
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CNTF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 92-100 | ||||||
| 1:2 | Average % | 90 | |||||
| Range % | 82-95 | ||||||
| 1:4 | Average % | 103 | |||||
| Range % | 99-110 | ||||||
| 1:8 | Average % | 87 | |||||
| Range % | 82-98 | ||||||
回收率：
| The recovery of rat CNTF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 82-95 | |||||
| EDTA plasma (n=4) | 108 | 106-113 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.1211.9852.0531.9135001.5981.5431.5711.4312501.2231.1441.1841.0441250.8650.9110.8880.748620.5420.5130.5280.38831.20.3390.3310.3350.19515.60.2570.2550.2560.11600.1420.1370.140 | |||||||
货期：3-5 working days
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