| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | RAI, RAI4, IASPP, NKIP1 |
| WB Predicted band size | 89 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human PPP1R13L |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PPP1R13L抗体的3篇参考文献，按文献名称、作者及摘要内容概括列出：
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1. **文献名称**: "PPP1R13L regulates the Ras signaling pathway and modulates cell survival"
**作者**: Li X, et al.
**摘要**: 该研究通过Western blot和免疫荧光技术验证了PPP1R13L抗体的特异性，发现PPP1R13L通过调控Ras通路抑制细胞凋亡，并在肺癌组织中高表达，提示其可能作为癌症治疗的潜在靶点。
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2. **文献名称**: "Antibody-based detection of PPP1R13L in colorectal cancer tissues"
**作者**: Wang Y, et al.
**摘要**: 研究利用商业化的PPP1R13L抗体对结直肠癌组织进行免疫组化分析，发现其表达水平与患者预后呈负相关，表明PPP1R13L可能作为肿瘤进展的生物标志物。
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3. **文献名称**: "Functional characterization of PPP1R13L in DNA damage response"
**作者**: Chen J, et al.
**摘要**: 通过siRNA敲低和PPP1R13L抗体验证实验，研究发现PPP1R13L参与DNA损伤修复过程，并与p53相互作用，调控细胞周期停滞和凋亡，揭示了其在基因组稳定性中的关键作用。
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注：以上文献信息为示例性内容，实际文献可能需要通过PubMed或Google Scholar以关键词“PPP1R13L antibody”或“RASSCF1A antibody”检索最新研究。
The PPP1R13L (Protein Phosphatase 1 Regulatory Subunit 13 Like) gene encodes a protein also known as RAI (RelA-associated inhibitor) or ASPP1 (apoptosis-stimulating protein of p53). This protein regulates key cellular processes, including apoptosis, stress response, and NF-κB/p53 signaling pathways. It interacts with PP1 phosphatase to modulate substrate dephosphorylation and acts as a context-dependent regulator of p53. enhancing p53-mediated apoptosis while inhibiting NF-κB activity. Dysregulation of PPP1R13L has been implicated in cancer, inflammation, and neurodegenerative diseases.
Antibodies targeting PPP1R13L are essential tools for studying its expression, localization, and functional roles. These antibodies are typically developed in hosts like rabbits or mice using immunogenic peptide sequences or recombinant protein fragments. They enable applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). Validation often includes knockout cell lines or siRNA knockdown to confirm specificity. Commercially available PPP1R13L antibodies vary in clonality (monoclonal/polyclonal) and epitope recognition (N-terminal, C-terminal, or internal regions). Researchers use these antibodies to explore PPP1R13L's dual role in tumor suppression (via p53 activation) and oncogenesis (through NF-κB inhibition), as well as its potential as a biomarker or therapeutic target in diseases like glioblastoma or colorectal cancer. Proper validation remains critical due to occasional cross-reactivity with homologous proteins like ASPP2.
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