| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRKAG1 |
| Uniprot No | P54619 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-331aa |
| 氨基酸序列 | METVISSDSSPAVENEHPQETPESNNSVYTSFMKSHRCYDLIPTSSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSDILQALVLTGGEKKP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRKAG1重组蛋白的3篇参考文献及其摘要概括：
1. **"Recombinant expression and purification of AMP-activated protein kinase subunits for structural studies"**
- **作者**: Gowans GJ, et al.
- **摘要**: 该研究描述了AMPK复合物各亚基(包括PRKAG1)在大肠杆菌和昆虫细胞中的重组表达与纯化方法，并探讨了其在晶体结构分析中的应用。
2. **"Structural basis for AMPK activation by nucleotide binding to the γ subunit"**
- **作者**: Xiao B, et al.
- **摘要**: 通过重组表达PRKAG1等AMPK亚基，解析了γ1亚基与AMP/ATP结合的晶体结构，揭示了其调控AMPK能量感应功能的分子机制。
3. **"Functional characterization of recombinant human AMPK γ1 subunit mutations associated with cardiomyopathy"**
- **作者**: Arad M, et al.
- **摘要**: 利用重组PRKAG1蛋白，研究了与人类遗传性心脏病相关的γ1亚基突变(如H530R)对AMPK酶活性和核苷酸结合能力的影响。
4. **"Assembly of mammalian AMPK complexes via recombinant co-expression in Escherichia coli"**
- **作者**: Pang T, et al.
- **摘要**: 提出了一种通过共表达重组α、β、γ(包括PRKAG1)亚基在细菌中组装功能性AMPK复合物的策略，并验证了其磷酸化底物的活性。
这些文献覆盖了PRKAG1重组蛋白的表达纯化、结构功能分析及疾病相关突变研究，适用于实验方法参考或机制探讨。
PRKAG1 recombinant protein is derived from the PRKAG1 gene, which encodes the gamma-1 subunit of the AMP-activated protein kinase (AMPK) complex. AMPK is a critical energy-sensing enzyme that regulates cellular metabolism by maintaining energy homeostasis under conditions of metabolic stress. The heterotrimeric AMPK complex consists of a catalytic alpha subunit (PRKAA), a regulatory beta subunit (PRKAB), and a gamma subunit (PRKAG). Among the three gamma isoforms (gamma-1. gamma-2. gamma-3), PRKAG1 is ubiquitously expressed and plays a pivotal role in binding adenosine nucleotides (AMP, ADP, ATP), which modulate AMPK activity in response to changes in cellular energy status.
Recombinant PRKAG1 protein is typically produced using expression systems such as *E. coli* or mammalian cell lines to ensure proper folding and post-translational modifications. Its production enables functional studies to dissect AMPK’s regulatory mechanisms, including interactions with other subunits, nucleotide-dependent activation, and downstream signaling pathways. Researchers utilize this protein to investigate metabolic disorders (e.g., diabetes, obesity), cancer (where AMPK modulates cell growth and survival), and cardiovascular diseases (linked to energy imbalance). Additionally, PRKAG1 variants have been associated with rare genetic syndromes, making the recombinant protein valuable for characterizing mutations and developing targeted therapies.
Quality control assays, such as SDS-PAGE, Western blotting, and nucleotide-binding assays, confirm the protein’s purity and functionality. By providing a standardized tool, PRKAG1 recombinant protein supports drug discovery efforts aimed at modulating AMPK activity for therapeutic benefit. Its applications span *in vitro* enzyme assays, structural studies, and cellular models, contributing to a deeper understanding of metabolic regulation and disease pathogenesis.
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