关键信息
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基因名
MAP2K4
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简介
The MAP2K4 protein is a dual-specificity kinase involved in the MAP kinase pathway. MAP2K4 Protein, Human (P. pastoris, His-GST) is the recombinant human-derived MAP2K4 protein, expressed by P. pastoris , with N-6*His, N-GST labeled tag.
- 应用
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种属
Human
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表达系统
P. pastoris
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标签
N-6*His;N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P45985
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表达区间
A2-D399
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氨基酸序列
AAPSPSGGGGSGGGSGSGTPGPVGSPAPGHPAVSSMQGKRKALKLNFANPPFKSTARFTLNPNPTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDLKDLGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPYIVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATPSSPMYVD
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蛋白长度
Full Length of Mature Protein
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
MAP2K4, also known as MKK4, is a dual-specificity mitogen-activated protein kinase kinase (MAPKK) that plays a significant role in the MAPK signaling pathways, specifically in the activation of c-Jun N-terminal kinases (JNK) and p38 MAPK. This protein is crucial for various cellular processes, including cell proliferation, differentiation, and apoptosis, making it a key player in the regulation of stress responses and inflammatory signaling. Mutations and dysregulation of MAP2K4 have been implicated in several pathological conditions, including cancer, neurodegenerative diseases, and inflammatory disorders. As a result, there has been increasing interest in understanding the functional dynamics of MAP2K4, particularly its role in tumorigenesis and cellular stress responses. Recent studies have focused on the structural characterization of MAP2K4 to elucidate its interaction with upstream regulators and downstream effectors. Additionally, the development of recombinant MAP2K4 proteins provides valuable tools for probing its biochemical properties and for exploring its potential as a therapeutic target. By generating and studying recombinant MAP2K4, researchers aim to gain insights into its regulatory mechanisms and to identify novel strategies for modulating its activity in various disease contexts. This research could pave the way for new therapeutic interventions targeting MAP2K4-related pathways, ultimately contributing to better diagnostic and treatment options for diseases involving aberrant MAPK signaling.
