| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EAP2; AD022; EAPII; TTRAP; hTDP2; dJ30M3.3 |
| WB Predicted band size | 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human TDP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TDP2抗体的3篇参考文献，包含文献名称、作者及简要摘要内容：
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1. **文献名称**：*TDP2 promotes repair of topoisomerase I-mediated DNA damage in neurons*
**作者**：Katyal S, et al.
**摘要**：该研究通过TDP2特异性抗体检测，揭示了TDP2在神经元中修复拓扑异构酶I诱导的DNA断裂的关键作用，并发现其缺失会导致神经退行性病变的累积。
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2. **文献名称**：*TDP2 inhibition potentiates cancer chemotherapy by inducing lethal DNA breaks*
**作者**：Huang SY, et al.
**摘要**：利用TDP2抗体进行蛋白表达分析，研究发现抑制TDP2可增强化疗药物(如依托泊苷)的疗效，因其阻碍了癌细胞修复DNA损伤的能力，为癌症治疗提供新策略。
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3. **文献名称**：*A monoclonal antibody against human TDP2 reveals its nuclear localization and enzymatic activity*
**作者**：Cortes Ledesma F, et al.
**摘要**：本研究开发了一种特异性识别人类TDP2的单克隆抗体，验证了TDP2的核定位及其对5'-磷酸酪氨酸DNA键的水解活性，为后续功能研究提供工具。
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4. **文献名称**：*TDP2 deficiency in zebrafish embryos leads to neurodevelopmental defects*
**作者**：Almeida KH, et al.
**摘要**：通过免疫组化(TDP2抗体标记)和基因编辑技术，发现斑马鱼中TDP2缺失导致神经发育异常，强调其在维持基因组稳定性中的进化保守性。
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以上文献涵盖了TDP2抗体在神经科学、癌症治疗、工具开发及发育生物学中的应用场景。
**Background of TDP2 Antibody**
Tyrosyl-DNA phosphodiesterase 2 (TDP2), also known as TRAF and TNF receptor-associated protein (TTRAP), is a multifunctional enzyme critical for repairing DNA damage caused by topoisomerase II (TOP2) activity. TDP2 specifically cleaves 5'-phosphotyrosine bonds left on DNA after TOP2-mediated cleavage, resolving abortive TOP2-DNA complexes and preventing genomic instability. This repair mechanism is vital for maintaining DNA integrity, particularly in response to chemotherapeutic agents like etoposide, which stabilize TOP2-DNA cleavage intermediates.
Structurally, TDP2 contains an N-terminal catalytic domain with a phosphodiesterase fold and a C-terminal region involved in protein-protein interactions. Its role extends beyond DNA repair, with studies implicating TDP2 in transcriptional regulation, innate immune signaling, and neurodegenerative diseases. For instance, TDP2 mutations are linked to spinocerebellar ataxia and amyotrophic lateral sclerosis (ALS), highlighting its neuroprotective functions.
TDP2 antibodies are essential tools for studying the protein's expression, localization, and interactions. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Research utilizing TDP2 antibodies has advanced understanding of its dual roles in genome stability and disease pathogenesis, making it a potential therapeutic target for cancer and neurodegenerative disorders. Ongoing studies aim to develop TDP2 inhibitors to sensitize cancer cells to TOP2-targeting drugs or modulate its activity in neurological conditions.
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