| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | EXTL |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human EXTL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EXTL1抗体的参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库查询确认)：
1. **文献名称**: *"EXTL1 regulates heparan sulfate biosynthesis and modulates Wnt signaling in development"*
**作者**: Kim, J. et al.
**摘要**: 本研究通过免疫沉淀和Western blot技术，利用EXTL1特异性抗体探究其在硫酸乙酰肝素合成中的作用，发现EXTL1缺失导致Wnt信号通路异常，影响胚胎发育。
2. **文献名称**: *"Expression profiling of EXTL1 in human cancers using a novel monoclonal antibody"*
**作者**: Tanaka, R. et al.
**摘要**: 研究开发了一种高特异性抗EXTL1单克隆抗体，并通过免疫组化分析其在多种肿瘤组织中的表达差异，提示EXTL1可能与肿瘤抑制功能相关。
3. **文献名称**: *"Functional interaction between EXT family proteins and EXTL1 revealed by co-immunoprecipitation assays"*
**作者**: Mizumoto, S. et al.
**摘要**: 利用EXTL1抗体进行共免疫沉淀实验，证实EXTL1与EXT1/EXT2蛋白形成复合物，共同调控糖胺聚糖链延伸，为遗传性骨病机制提供新见解。
4. **文献名称**: *"EXTL1 antibody-based detection in zebrafish models highlights its role in angiogenesis"*
**作者**: Chen, L. et al.
**摘要**: 通过斑马鱼模型和免疫荧光技术，结合EXTL1抗体，揭示其在血管生成中的关键作用，为心血管疾病研究提供潜在靶点。
建议通过PubMed或Google Scholar输入“EXTL1 antibody”或“EXTL1 function”获取具体文献全文及验证信息。
The EXTL1 antibody is a research tool designed to detect and study the exostosin-like 1 (EXTL1) protein, a member of the EXT/EXTL family of glycosyltransferases. EXTL1 is implicated in heparan sulfate (HS) biosynthesis, a critical process for forming heparan sulfate proteoglycans (HSPGs), which regulate cell signaling, adhesion, and extracellular matrix interactions. Unlike its homologs EXT1 and EXT2. which form a heterocomplex essential for HS chain elongation, EXTL1's precise role remains less defined. Studies suggest it may act as a chain initiator or modifier in HS synthesis, though its enzymatic activity and substrates are still under investigation.
The EXTL1 antibody is commonly used in applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to analyze protein expression, localization, and interactions in various tissues and cell types. It has been employed in cancer research, as dysregulation of HSPGs is linked to tumor progression, angiogenesis, and metastasis. Additionally, EXTL1 mutations or altered expression have been associated with skeletal disorders and inherited conditions, though these links are less well-characterized compared to EXT1/EXT2.
Researchers rely on validated EXTL1 antibodies to explore its biological functions, particularly in developmental biology and disease models. Specificity and cross-reactivity with other EXTL family members (e.g., EXTL2. EXTL3) should be confirmed via knockout controls or siRNA-based validation to ensure accurate interpretation of results. Commercial EXTL1 antibodies are typically raised against peptide sequences unique to the protein, with reactivity across species like human, mouse, and rat.
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