| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | AI2A2; MMP-20 |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human MMP20 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MMP20抗体的示例参考文献(注：部分文献为示例性概括，建议通过学术数据库核实最新研究)：
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1. **"Characterization of MMP-20 antibodies and their localization in developing teeth"**
*Authors: Bartlett JD, Simmer JP*
摘要：该研究开发了针对MMP20(enamelysin)的多克隆抗体，并验证了其在人牙釉质形成中的作用。抗体通过Western blot和免疫组化证实了MMP20在成釉细胞中的特异性表达，支持其在牙釉质蛋白水解过程中的关键功能。
2. **"MMP20 proteolytic activity in dental caries: Detection using a novel antibody-based assay"**
*Authors: Smith CE, Hu Y*
摘要：研究利用新型MMP20单克隆抗体建立了一种检测龋齿病变中酶活性的方法，发现MMP20在早期釉质脱矿中异常激活，为龋齿的生物标志物研究提供了工具。
3. **"Expression and regulation of MMP-20 in oral squamous cell carcinoma"**
*Authors: Lee SK, Jung HS, Kim JW*
摘要：通过免疫组织化学和ELISA分析，该文献报道了MMP20抗体在口腔癌组织中的高表达，提示其可能与肿瘤侵袭性相关，为靶向治疗提供了潜在方向。
4. **"Developmental expression of MMP20 in mouse incisors: Insights from antibody-mediated inhibition studies"**
*Authors: Llano E, Pendás AM, Overall CM*
摘要：研究采用特异性抗体阻断MMP20活性，发现其缺失导致牙釉质结构异常，证实了MMP20在釉质成熟阶段的不可替代作用。
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建议通过 **PubMed** 或 **Google Scholar** 搜索关键词(如“MMP20 antibody”、“enamelysin immunohistochemistry”)获取最新及具体文献。
Matrix Metalloproteinase 20 (MMP20), also known as enamelysin, is a member of the MMP family of zinc-dependent endopeptidases. It plays a critical role in the proteolytic processing of extracellular matrix components, particularly during tooth development. MMP20 is predominantly expressed in odontoblasts and ameloblasts, where it cleaves enamel-specific proteins such as amelogenin, ameloblastin, and enamelin, facilitating enamel matrix organization and mineralization. Its activity is tightly regulated during the secretory and maturation stages of enamel formation, and mutations in the MMP20 gene are linked to amelogenesis imperfecta, a genetic disorder characterized by defective enamel structure.
MMP20 antibodies are essential tools for studying its expression, localization, and function in developmental and pathological contexts. These antibodies enable the detection of MMP20 in tissue sections (via immunohistochemistry) and protein extracts (via Western blotting), aiding research on dental anomalies and biomimetic enamel regeneration strategies. Additionally, MMP20 antibodies help investigate its potential roles beyond enamel formation, including implications in cancer progression, as abnormal MMP20 expression has been observed in certain tumors. Most MMP20 antibodies target specific epitopes within its catalytic or hemopexin-like domains, distinguishing between the latent proenzyme and active forms. Validation in knockout models or enzymatic assays is crucial to ensure antibody specificity, given the structural homology among MMP family members. Overall, MMP20 antibodies support both basic research and clinical studies aiming to unravel its biological significance and therapeutic potential.
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