| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ADORA2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ADORA2B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于ADORA2B抗体的代表性文献(内容基于领域内常见研究方向概括)：
1. **文献名称**：*Adenosine A2B receptor signaling in hypoxia-induced pulmonary hypertension*
**作者**：Zhang Y. et al.
**摘要**：本研究利用ADORA2B特异性抗体，通过免疫组化和Western blot技术，揭示了A2B受体在缺氧诱导的肺动脉高压模型中的表达上调，并证明其通过调控炎症因子释放参与疾病进程。
2. **文献名称**：*ADORA2B-mediated adenosine signaling promotes age-related cognitive decline*
**作者**：Li H. et al.
**摘要**：作者通过ADORA2B抗体标记发现，老年小鼠海马区A2B受体表达显著增加，基因敲除或抗体阻断该受体可改善记忆功能，提示其作为阿尔茨海默病潜在干预靶点。
3. **文献名称**：*Development and validation of a high-affinity monoclonal antibody for ADORA2B detection*
**作者**：Müller C.E. et al.
**摘要**：该文献报道了一种新型ADORA2B单克隆抗体的开发过程，通过流式细胞术和免疫荧光验证其特异性，为受体亚型定位研究提供了高灵敏度工具。
(注：以上文献为示例性概括，实际引用需以具体发表的论文信息为准。)
The ADORA2B antibody targets the adenosine A2B receptor, a G-protein-coupled receptor (GPCR) that binds adenosine, a purine nucleoside involved in cellular energy homeostasis and signaling. ADORA2B, encoded by the ADORA2B gene, is one of four adenosine receptor subtypes (A1. A2A, A2B, A3) and is distinguished by its lower affinity for adenosine compared to A1 and A2A. It couples primarily to Gs proteins, activating adenylyl cyclase to increase intracellular cAMP, though it may also engage Gq-mediated pathways under certain conditions.
ADORA2B is widely expressed in vascular endothelial cells, immune cells (e.g., mast cells, macrophages), and the central nervous system. It plays roles in vasodilation, inflammation, hypoxia adaptation, and fibrosis. Research highlights its involvement in pathological conditions such as ischemic injury, chronic lung diseases, and cancer progression, where it modulates immune responses and angiogenesis.
ADORA2B antibodies are essential tools for studying receptor localization, expression levels, and signaling mechanisms. They are used in techniques like Western blotting, immunohistochemistry, and flow cytometry to explore ADORA2B's functional contributions in disease models. Recent studies also investigate its potential as a therapeutic target, with antagonists or agonists being explored for treating inflammatory disorders, cardiovascular diseases, or cancer. Validated ADORA2B antibodies typically exhibit specificity for human, mouse, or rat isoforms, enabling cross-species translational research.
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