首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人桥粒芯蛋白3(DSG3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DSG3含量。DSG3（桥粒芯糖蛋白 3）是一种重要的细胞黏附分子，在维持上皮组织完整性方面发挥关键作用。研究发现其异常表达与多种疾病尤其是天疱疮密切相关，机制上可能涉及自身抗体与 DSG3 结合，破坏细胞间黏附，为相关疾病研究和治疗提供了靶点思路。试剂盒检测范围为28 pg/mL-1800 pg/mL，适用于科研领域研究DSG3在皮肤屏障功能、上皮组织损伤修复或自身免疫反应中的动态变化，例如通过检测疾病模型或实验干预后样本中的DSG3水平，揭示其在细胞黏附调控及病理过程中的作用，为分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：130 kD pemphigus vulgaris antigen ELISA Kit; 130 kDa pemphigus vulgaris antigen ELISA Kit; Balding ELISA Kit; Cadherin family member 6 ELISA Kit; CDHF 6 ELISA Kit; CDHF6 ELISA Kit; Desmoglein 3 ELISA Kit; Desmoglein 3 precursor ELISA Kit; Desmoglein-3 ELISA Kit; Desmoglein3 ELISA Kit; DKFZp686P23184 ELISA Kit; DSG 3 ELISA Kit; DSG3 ELISA Kit; DSG3_HUMAN ELISA Kit; Pemphigus vulgaris antigen ELISA Kit; PVA ELISA Kit
缩写：DSG3
Uniprot No.：P32926
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：28 pg/mL-1800 pg/mL
灵敏度：7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DSG3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-105 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of human DSG3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-97 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected18002.8262.7782.8022.6969002.0902.0002.0451.9394501.1571.1641.1611.0552250.5180.4980.5080.4021120.3350.3230.3290.223560.2190.2140.2170.111280.1470.1420.1450.03900.1050.1070.106 | ||||||
货期：3-5 working days
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