首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人桥粒芯糖蛋白-1(Dsg-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的DSG1含量。DSG1属桥粒芯糖蛋白家族，是细胞间连接蛋白。它在皮肤屏障形成和维持中起重要作用，其表达异常与多种皮肤疾病相关。研究聚焦于其在细胞黏附、信号传导机制，探索以DSG1为靶点开发治疗皮肤疾病的药物。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于体外实验中对Dsg - 1表达变化的动态分析，如皮肤病理机制研究、药物作用靶点筛选或细胞间连接相关分子调控的探索，为皮肤生物学、免疫学及疾病模型构建等科研领域提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DSG1; CDHF4; Desmoglein-1; Cadherin family member 4; Desmosomal glycoprotein 1; DG1; DGI; Pemphigus foliaceus antigen
缩写：DSG1
Uniprot No.：Q02413
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Dsg-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 92-102 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 86-98 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of human Dsg-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-96 | ||||
| EDTA plasma (n=4) | 87 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.3082.5122.4102.2857501.3421.4451.3941.2693750.7790.7570.7680.643187.50.4430.4570.4500.325940.3120.3030.3080.183470.2310.2340.2330.10823.50.1980.1920.1950.07000.1240.1250.125 | ||||||
货期：3-5 working days
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