首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠R脊椎蛋白3(RSPO3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RSPO3含量。试剂盒检测范围为4.7 pg/mL-300 pg/mL，可广泛应用于科研领域，如探究小鼠疾病模型中RSPO3的表达调控机制、评估基因编辑或药物干预对靶蛋白水平的影响，以及组织微环境中RSPO3与相关信号通路的关联性研究，为肿瘤生物学、再生医学等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Rspo3 ELISA Kit; R-spondin-3 ELISA Kit; Cabriolet ELISA Kit; Cysteine-rich and single thrombospondin domain-containing protein 1 ELISA Kit; Cristin-1 ELISA Kit; Nucleopondin ELISA Kit; Roof plate-specific spondin-3 ELISA Kit
缩写：RSPO3
Uniprot No.：Q2TJ95
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：4.7 pg/mL-300 pg/mL
灵敏度：1.17 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Developmental Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse RSPO3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-105 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 92-96 | |||||
回收率：
| The recovery of mouse RSPO3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 94-100 | ||||
| EDTA plasma (n=4) | 93 | 90-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected3002.6932.8002.7472.5971501.8111.9701.8911.741751.0731.1351.1040.95437.50.6520.7010.6770.52718.750.3690.3750.3720.2229.40.2470.2660.2570.1074.70.2020.2140.2080.05800.1580.1420.150 | ||||||
货期：3-5 working days
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