| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF75A |
| Uniprot No | Q96N20 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 58-162aa |
| 氨基酸序列 | SPEFKDSAGKSPTGLKLKNDTENHQPVSLSDLEIQASAGVISKKAKVKVP QKTAGKENHFDMHRVGKWHQDFPVKKRKKLSTWKQELLKLMDRHKKDCAR EKPFK |
| 预测分子量 | 12 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设的模拟参考文献示例，供参考。由于ZNF75A的研究相对有限，建议通过PubMed或Google Scholar检索最新文献：
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1. **标题**：*"Recombinant Expression and DNA-Binding Properties of Human ZNF75A Protein"*
**作者**：Zhang L, et al.
**摘要**：本研究在大肠杆菌系统中成功表达并纯化了ZNF75A重组蛋白，通过电泳迁移率实验(EMSA)证实其特异性结合GC-rich DNA序列，提示其在基因转录调控中的潜在作用。
2. **标题**：*"ZNF75A Recombinant Protein Suppresses Tumor Growth via Modulating Oncogenic Pathways"*
**作者**：Wang Y, et al.
**摘要**：利用哺乳动物细胞表达的重组ZNF75A蛋白，研究发现其通过抑制c-Myc转录活性，降低癌细胞增殖，为癌症治疗提供了新靶点。
3. **标题**：*"Structural Characterization of ZNF75A Zinc Finger Domain by X-ray Crystallography"*
**作者**：Patel R, et al.
**摘要**：解析了ZNF75A锌指结构域的晶体结构，揭示了其与DNA相互作用的分子机制，为设计锌指蛋白工具提供结构基础。
4. **标题**：*"Functional Screening of ZNF75A Isoforms Using Recombinant Protein Libraries"*
**作者**：Kim S, et al.
**摘要**：构建了ZNF75A不同剪接变体的重组蛋白库，发现某异构体在神经元分化中显著激活特定基因表达，提示功能多样性。
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**注意**：以上为模拟示例，实际文献需通过学术数据库检索。可尝试关键词：`ZNF75A recombinant protein`、`ZNF75A expression` 或 `ZNF75A function`。
ZNF75A (Zinc Finger Protein 75A), also known as ZNF75 or ZNF82. is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family. These proteins are characterized by tandem C2H2-type zinc finger motifs, which facilitate sequence-specific DNA binding, and a KRAB domain at the N-terminus that often mediates transcriptional repression through interactions with co-repressor complexes. ZNF75A is encoded by a primate-specific gene located on chromosome 16q22.1 and is predominantly expressed in the nucleus, suggesting roles in transcriptional regulation and chromatin remodeling.
Functionally, ZNF75A is implicated in modulating gene expression networks, particularly in cell differentiation, proliferation, and stress responses. Studies link it to epigenetic regulation through associations with histone deacetylases (HDACs) or other chromatin-modifying enzymes. However, its precise biological mechanisms and target genes remain incompletely characterized, partly due to functional redundancy among zinc finger proteins.
Recombinant ZNF75A protein is typically produced in Escherichia coli or mammalian expression systems, enabling biochemical and structural studies. The purified protein often retains its DNA-binding capacity and is used to investigate interactions with nucleic acids or protein partners. Common applications include chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assays (EMSAs), and in vitro transcriptional assays. Researchers also employ ZNF75A recombinant proteins to study its role in diseases, as altered expression has been observed in cancers and neurological disorders. Current challenges in ZNF75A research include mapping its genome-wide binding sites and resolving its three-dimensional structure to inform therapeutic targeting strategies.
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