| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HEP-NOS; NOS2; inducible; |
| Entrez GeneID | 4843; |
| WB Predicted band size | 131kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 151(Q-Y-Y(p)-G-S) derived from Human iNOS . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与iNOS (Phospho-Tyr151)抗体相关的文献概览(注：具体文献需根据实际数据库验证，以下为模拟示例)：
1. **文献名称**：*Tyrosine phosphorylation of iNOS regulates inflammatory responses in macrophages*
**作者**：Smith A et al.
**摘要**：研究揭示了iNOS在Tyr151位点的磷酸化通过调控其酶活性和亚细胞定位，影响巨噬细胞中一氧化氮(NO)的生成，进而调节炎症反应。该文献使用Phospho-Tyr151抗体验证了磷酸化修饰的存在。
2. **文献名称**：*Role of iNOS phosphorylation in sepsis-induced vascular dysfunction*
**作者**：Chen L et al.
**摘要**：通过特异性Phospho-Tyr151抗体检测，发现脓毒症模型中iNOS的Tyr151磷酸化水平显著升高，导致血管内皮细胞功能紊乱，提示该位点可能是治疗脓毒症的潜在靶点。
3. **文献名称**：*JAK2 mediates iNOS activation via tyrosine phosphorylation in asthma*
**作者**：Kim Y et al.
**摘要**：研究证明JAK2激酶通过诱导iNOS Tyr151位点的磷酸化，促进气道炎症中NO的过量产生，Phospho-Tyr151抗体被用于Western blot和免疫组化分析。
**说明**：以上文献标题及内容为示例性质，实际文献需通过PubMed、Web of Science等平台以关键词“iNOS Phospho-Tyr151”或“iNOS tyrosine 151 phosphorylation”检索确认。部分研究可能涉及该抗体的应用(如检测磷酸化水平)或机制探讨(如信号通路调控)。
iNOS (inducible nitric oxide synthase), also known as NOS2. is an enzyme responsible for producing nitric oxide (NO) in response to inflammatory or immunological stimuli. Its activity is tightly regulated at transcriptional and post-translational levels. Phosphorylation at tyrosine residues, including Tyr151. represents a key regulatory mechanism influencing iNOS stability, localization, and enzymatic function. The Tyr151 phosphorylation site has been implicated in modulating interactions with regulatory proteins and downstream signaling pathways, particularly in macrophages and other immune cells.
The iNOS (Phospho-Tyr151) antibody is a specialized tool designed to detect the phosphorylated form of iNOS at this specific residue. It enables researchers to study post-translational modifications of iNOS under conditions like inflammation, infection, or oxidative stress. This antibody finds applications in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate iNOS activation dynamics in disease models including sepsis, atherosclerosis, and cancer. Its specificity helps distinguish between basal and activated iNOS states, providing insights into NO-mediated pathological processes. Validation typically includes testing in phosphorylated vs. non-phosphorylated protein samples and genetic/pharmacological modulation of phosphorylation pathways. Understanding iNOS phosphorylation mechanisms may reveal therapeutic targets for conditions involving dysregulated NO signaling.
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