| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HECH; HP1 gamma; HP1Hs-gamma; chromobox 3; |
| Entrez GeneID | 11335; |
| WB Predicted band size | 24kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of Serine 93(R-L-S(p)-L-S) derived from Human HP1γ . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HP1γ (Phospho-Ser93)抗体的假设性参考文献示例(实际文献需通过学术数据库验证)：
1. **"Phosphorylation of HP1γ at Ser93 regulates heterochromatin assembly in mitosis"**
- 作者：Smith A et al.
- 摘要：研究揭示了HP1γ的Ser93磷酸化在细胞有丝分裂过程中调控异染色质动态的机制，通过该抗体验证磷酸化水平与染色质结合的负相关性。
2. **"Dynamic phosphorylation of HP1γ modulates its interaction with histone H3K9me3"**
- 作者：Li X et al.
- 摘要：利用Phospho-Ser93抗体进行免疫共沉淀实验，证明Ser93磷酸化削弱HP1γ与H3K9me3的结合，影响基因沉默功能。
3. **"Phospho-Ser93 HP1γ as a biomarker for DNA damage response in cancer cells"**
- 作者：Wang Y et al.
- 摘要：通过Western blot和免疫荧光分析，发现电离辐射诱导的Ser93磷酸化与HP1γ在DNA损伤修复中的重新定位相关。
4. **"Role of HP1γ phosphorylation in epigenetic regulation during cellular differentiation"**
- 作者：Garcia R et al.
- 摘要：使用Phospho-Ser93抗体探究该修饰在干细胞分化中对染色质重塑的影响，提示其通过调控HP1γ与染色质解离参与分化进程。
**注意**：以上内容为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词“HP1γ Phospho-Ser93 antibody”或“HP1γ Ser93 phosphorylation”获取。
The HP1γ (Phospho-Ser93) antibody is a specialized tool used to study the post-translational modification of Heterochromatin Protein 1 gamma (HP1γ, also called CBX3), a key component of chromatin organization. HP1 proteins, including HP1α, HP1β, and HP1γ, are characterized by a conserved chromo domain that binds to histone H3 trimethylated at lysine 9 (H3K9me3), facilitating heterochromatin formation and gene silencing. HP1γ, unlike its isoforms, is enriched in euchromatin and participates in transcriptional regulation, DNA repair, and chromatin dynamics.
Phosphorylation at Ser93 is a critical regulatory modification of HP1γ. This site-specific phosphorylation, often induced during mitosis or stress responses, modulates HP1γ’s interaction with chromatin and other proteins. Studies suggest that phosphorylation at Ser93 disrupts HP1γ’s binding to H3K9me3. promoting its release from chromatin during mitosis to enable chromatin relaxation and cell cycle progression. The modification is mediated by kinases such as Aurora B, linking it to cell division and epigenetic plasticity.
The HP1γ (Phospho-Ser93) antibody specifically detects this phosphorylated form, enabling researchers to explore its role in cell cycle regulation, chromatin remodeling, and diseases like cancer, where aberrant HP1γ phosphorylation may correlate with genomic instability or altered gene expression. Applications include Western blotting, immunofluorescence, and ChIP assays. Validation typically involves knockdown/knockout controls or phosphatase treatment to confirm signal specificity. This antibody serves as a vital reagent for dissecting the dynamic interplay between epigenetic regulation and cellular processes.
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