| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAK; RTK6; TRKE; EDDR1; |
| Entrez GeneID | 780; |
| WB Predicted band size | 110kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 513 (K-K-Y(p)-V-R) derived from Human DDR1 . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DDR1 (Phospho-Tyr513)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Structural and functional characterization of DDR1 phosphorylation"*
**作者**: Vogel W, et al.
**摘要**: 该研究解析了DDR1受体的磷酸化位点(包括Tyr513)，通过Western blot和免疫沉淀实验验证了Phospho-Tyr513抗体的特异性，并证明其在胶原结合后触发下游信号通路中的关键作用。
2. **文献名称**: *"Collagen-induced DDR1 activation requires phosphorylation at Tyr513"*
**作者**: Leitinger B, et al.
**摘要**: 研究发现胶原蛋白激活DDR1依赖于Tyr513位点的磷酸化，利用Phospho-Tyr513抗体检测到该修饰在细胞迁移和基质重塑中的动态变化，为靶向DDR1信号提供依据。
3. **文献名称**: *"DDR1 phosphorylation in tumor-associated macrophages promotes immunosuppressive microenvironment"*
**作者**: Mohammadpour H, et al.
**摘要**: 通过免疫组化与Phospho-Tyr513抗体，揭示了DDR1在肿瘤微环境中的磷酸化状态与免疫抑制相关，为癌症治疗中靶向DDR1磷酸化提供新视角。
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注：以上文献为示例，实际引用需以具体发表文章为准。若需实验级抗体验证文献，建议检索PubMed等数据库，结合“DDR1”、“phosphorylation”、“antibody validation”等关键词筛选。
The DDR1 (Phospho-Tyr513) antibody is a specialized tool used to detect the phosphorylated tyrosine 513 residue on the Discoidin Domain Receptor 1 (DDR1), a receptor tyrosine kinase (RTK) activated by collagen binding. DDR1 plays critical roles in cell adhesion, proliferation, and extracellular matrix remodeling by mediating collagen-induced signaling. Upon ligand binding, DDR1 undergoes autophosphorylation at specific tyrosine residues, including Tyr513. which serves as a docking site for downstream adaptor proteins, initiating signaling cascades like MAPK, PI3K/Akt, and NF-κB pathways. Phosphorylation at Tyr513 is essential for DDR1's activation and its involvement in physiological processes (e.g., tissue development) and pathological conditions (e.g., fibrosis, cancer metastasis, inflammation).
This antibody is widely used in research to study DDR1 activation dynamics in disease models, particularly cancers (breast, lung, glioblastoma) and fibrotic disorders, where DDR1 overexpression or hyperactivation is observed. It enables specific detection of the active, phosphorylated form of DDR1 via techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Researchers rely on its specificity to distinguish phosphorylated DDR1 from non-phosphorylated states or other RTKs, ensuring accurate assessment of DDR1 signaling activity. Proper experimental controls (e.g., phosphatase-treated samples) are recommended to validate results. The antibody thus serves as a key reagent in elucidating DDR1's role in cellular responses to collagen and its therapeutic targeting.
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