Rabbit Polyclonal Daxx(Phospho-Ser668) Antibody

中文名： Daxx (Phospho-Ser668)抗体
别名： DAXX; BING2; DAP6; Death domain-associated protein 6; Daxx; hDaxx; ETS1-associated protein 1; EAP1; Fas death domain-associated protein

货号: IPDX40892

Price： ￥1280

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/100-1/300	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	1/10000	Human,Mouse,Rat

产品详情

Aliases	DAXX; BING2; DAP6; Death domain-associated protein 6; Daxx; hDaxx; ETS1-associated protein 1; EAP1; Fas death domain-associated protein
Entrez GeneID	1616;
WB Predicted band size	81kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized peptide derived from human Daxx around the phosphorylation site of S668.
Formulation	Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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Western Blot analysis of 293 cells using Phospho-Daxx (S668) Polyclonal Antibody
Immunohistochemical analysis of paraffin-embedded human ovary using P40892 at dilution 1/100 at the left, antigen peptide blocked at the right

参考文献

以下是关于Daxx (Phospho-Ser668)抗体的3篇参考文献示例(文献信息为示例性质，实际引用时建议通过数据库核实)：

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1. **文献名称**：*HIPK1 phosphorylates DAXX to trigger oxidative stress-dependent necroptosis in glioblastoma*

**作者**：Hofmann TG, et al.

**摘要**：研究揭示了HIPK1激酶通过磷酸化Daxx的Ser668位点(使用Phospho-Ser668特异性抗体验证)，在氧化应激条件下激活坏死性凋亡通路，为胶质母细胞瘤的治疗提供新靶点。

2. **文献名称**：*Phosphorylation of DAXX by ATM contributes to DNA damage-induced activation of the apoptotic pathway*

**作者**：Tian Y, et al.

**摘要**：本文发现DNA损伤后，ATM激酶介导Daxx的Ser668位点磷酸化(通过Phospho-Ser668抗体检测)，促进Daxx从核内PML小体转位至胞质，进而激活下游凋亡信号通路。

3. **文献名称**：*Serine 668 phosphorylation enhances DAXX-mediated apoptosis through interaction with ASK1*

**作者**：Jin C, et al.

**摘要**：研究证明Daxx的Ser668磷酸化(通过特异性抗体验证)增强了其与凋亡信号调节激酶ASK1的结合能力，从而在TNF-α刺激下放大JNK/p38通路介导的细胞凋亡。

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**说明**：以上文献为基于Daxx磷酸化相关研究的概括性示例，实际研究中建议通过PubMed或Google Scholar以关键词“Daxx Ser668 phosphorylation”或“Daxx phospho-Ser668 antibody”检索最新文献，并关注抗体供应商(如CST、Abcam)的技术文档以获取具体引用信息。

背景信息

The Daxx (Phospho-Ser668) antibody detects the phosphorylation of the Death Domain-associated protein 6 (DAXX) at serine residue 668. a post-translational modification linked to its functional regulation. DAXX is a multifunctional protein involved in diverse cellular processes, including apoptosis, transcriptional regulation, chromatin remodeling, and stress response. It primarily localizes to the nucleus, where it interacts with PML bodies, but can translocate to the cytoplasm under specific stimuli. Phosphorylation at Ser668. mediated by kinases such as Akt or PKC, modulates DAXX's subcellular distribution and protein interactions. This modification has been implicated in cancer progression, particularly in contexts like prostate cancer and glioblastoma, where it may influence cell survival, DNA damage response, or TGF-β signaling pathways.

The Daxx (Phospho-Ser668) antibody is widely used in research to study DAXX's role in signaling cascades and disease mechanisms. It helps assess phosphorylation status in experimental models, such as cells treated with growth factors, stressors, or kinase inhibitors. Detection of this modification provides insights into DAXX's regulatory mechanisms and its potential as a biomarker or therapeutic target. Validation often includes Western blotting, immunofluorescence, or immunohistochemistry in phosphorylated DAXX-specific contexts. Understanding Ser668 phosphorylation is critical for unraveling DAXX's dual roles in tumor suppression and oncogenesis, depending on cellular context and interaction partners.