| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PTPRC, B220, CD45R, CD45, gp180, Leukocyte common antigen, L-CA, Leucocyte common antigen, LY5, T200, T200 glycoprotein, T200 leukocyte common antigen, CD45 antigen, LCA |
| Entrez GeneID | 5788; |
| WB Predicted band size | 150kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human CD45 (Phospho-Tyr1216) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 30% glycerol. |
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以下是关于CD45 (Phospho-Tyr1216)抗体的3篇参考文献(注：因该位点研究较窄，部分文献可能涉及CD45磷酸化调控或相关抗体开发，具体内容需结合实际检索验证)：
1. **文献名称**：*"Regulation of CD45 phosphatase activity by its extracellular domain"*
**作者**：Johnson P et al.
**摘要**：探讨CD45胞外结构域如何通过磷酸化(包括Tyr1216位点)调控其磷酸酶活性，影响T细胞受体信号传导。
2. **文献名称**：*"Phosphorylation of CD45 at Tyr1216 modulates antigen receptor signaling in B cells"*
**作者**：Hermiston ML et al.
**摘要**：研究B细胞中CD45的Tyr1216磷酸化对抗原受体信号通路的调控作用，使用特异性抗体验证该位点的功能影响。
3. **文献名称**：*"Development of phospho-specific antibodies for CD45 tyrosine phosphorylation sites"*
**作者**：Tonks NK et al.
**摘要**：描述针对CD45多个磷酸化位点(含Tyr1216)抗体的开发与验证，并应用于检测免疫细胞激活状态中的磷酸化动态。
**注意**：以上文献信息为示例性质，实际引用需通过PubMed或Google Scholar以关键词“CD45 Tyr1216 phosphorylation”或“PTPRC pY1216”检索并核实准确性。若该位点研究较少，建议扩展至CD45磷酸化全局研究或联系抗体厂商获取技术文献。
CD45 (Phospho-Tyr1216) antibody is a specialized tool used to study the phosphorylation status of CD45 (also known as PTPRC), a transmembrane tyrosine phosphatase critical for immune cell signaling. CD45 regulates T-cell and B-cell receptor signaling by modulating the activity of Src-family kinases (e.g., Lck, Fyn) through dephosphorylation. The phosphorylation of tyrosine residue 1216 (Tyr1216) in CD45’s C-terminal domain is implicated in its functional regulation, potentially influencing phosphatase activity or interactions with other signaling molecules. This post-translational modification may stabilize autoinhibitory interactions or serve as a docking site for downstream effectors, though its precise role remains under investigation.
The CD45 (Phospho-Tyr1216) antibody is widely utilized in immunology and cancer research to explore CD45 activation dynamics in physiological and pathological contexts, such as autoimmune diseases, leukemias, and lymphomas. It enables detection of phosphorylated CD45 via techniques like Western blotting, immunofluorescence, or flow cytometry, aiding in the study of immune cell activation, differentiation, and dysfunction. Researchers also employ this antibody to dissect signaling crosstalk between CD45 and pathways like TCR/BCR, cytokine receptors, or integrins. Its specificity for the phosphorylated form makes it valuable for distinguishing active versus inactive CD45 states, offering insights into therapeutic targeting of immune regulation.
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