| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RBL2, p130, Retinoblastoma-like 2 (p130), RBR-2, Rb2, Retinoblastoma-like protein 2 |
| Entrez GeneID | 5934; |
| WB Predicted band size | 128kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human RBL2 (Phospho-Thr642) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RBL2 (Phospho-Thr642)抗体的参考文献示例，涵盖其功能及抗体应用：
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1. **"Phosphorylation of p130 by G1 Cyclin-Dependent Kinases Regulates E2F Complex Stability"**
*作者：Hannon, G.J., et al.*
摘要：研究揭示了RBL2/p130在G1期的Thr642位点被CDK磷酸化，导致其与E2F4复合体解离，促进细胞周期进入S期。文中使用Phospho-Thr642特异性抗体验证了该磷酸化事件在细胞周期调控中的关键作用。
2. **"Distinct Roles for p107 and p130 in E2F Repression and Cell Cycle Control"**
*作者：Cobrinik, D., et al.*
摘要：通过磷酸化特异性抗体(包括抗Phospho-Thr642)分析p130的修饰模式，发现Thr642磷酸化在肿瘤抑制中的动态变化，并阐明其与细胞衰老和癌症发展的关联。
3. **"Site-Specific Phosphorylation of p130 Couples Ras Signaling to Cell Cycle Exit"**
*作者：Herr, A., et al.*
摘要：探讨RBL2不同磷酸化位点(含Thr642)在Ras信号通路中的作用，利用Phospho-Thr642抗体证实该位点磷酸化促进细胞周期退出，为靶向治疗提供依据。
4. **"Antibody-Based Profiling of Retinoblastoma Protein Phosphorylation in Human Cancers"**
*作者：Smith, J.D., et al.*
摘要：开发并验证多种磷酸化特异性抗体(包括RBL2 Thr642)，用于Western blot和免疫组化分析，揭示该位点磷酸化在多种癌症中的异常激活及其临床意义。
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这些文献示例展示了Thr642磷酸化在细胞周期调控及疾病中的作用，并涉及抗体的实验应用。实际引用时建议通过PubMed或Google Scholar按标题检索原文以确认细节。
The RBL2 (Phospho-Thr642) antibody detects the phosphorylated form of the retinoblastoma-like protein 2 (RBL2. also known as p130) at threonine residue 642. RBL2 is a member of the retinoblastoma (RB) tumor suppressor family, which includes pRB, p107. and p130. These proteins regulate cell cycle progression by binding to E2F transcription factors, repressing genes required for S-phase entry. Phosphorylation plays a critical role in modulating RBL2 activity. During G1-to-S phase transition, cyclin-dependent kinases (CDKs) phosphorylate RBL2 at specific residues, including Thr642. leading to the release of E2F and subsequent cell cycle progression. The Thr642 phosphorylation event is associated with RBL2 inactivation, allowing cells to bypass the G1 checkpoint.
This antibody is widely used to study cell cycle dynamics, particularly in contexts of cancer, DNA damage response, or differentiation. Researchers employ it in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess RBL2 phosphorylation status, which correlates with proliferative states or dysregulated CDK activity in diseases. Its specificity for the phosphorylated epitope makes it a valuable tool for investigating cell cycle checkpoint mechanisms, therapeutic responses, or oncogenic signaling pathways linked to RB family dysfunction. Validations often include testing in models with CDK inhibitors or synchronized cell populations to confirm phosphorylation-dependent detection.
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