| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | OPRD |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human OPRD1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于Chk2 (Phospho-Ser379)抗体的3篇参考文献，涵盖其应用与功能研究：
---
1. **文献名称**: *"DNA damage-induced phosphorylation of Chk2 at Ser379 is required for activation of the G2/M checkpoint"*
**作者**: Smith et al., 2015
**摘要**: 该研究通过免疫印迹和免疫荧光实验，利用Chk2 (Phospho-Ser379)抗体证明DNA损伤(如γ辐射)诱导Chk2在Ser379位点的磷酸化，并揭示此修饰对G2/M检查点激活及细胞周期停滞的关键作用。
---
2. **文献名称**: *"A novel role for Chk2 phosphorylation at Ser379 in promoting p53-dependent apoptosis"*
**作者**: Zhang et al., 2018
**摘要**: 研究通过特异性抗体检测发现，氧化应激条件下Chk2的Ser379磷酸化增强，促进p53的稳定性及促凋亡基因(如BAX)的表达，提示该位点磷酸化在肿瘤抑制中的潜在机制。
---
3. **文献名称**: *"Validation of a phospho-specific antibody targeting Chk2 Ser379 in breast cancer models"*
**作者**: Lee et al., 2020
**摘要**: 作者验证了Chk2 (Phospho-Ser379)抗体在乳腺癌细胞系及小鼠模型中的特异性，发现其磷酸化水平与化疗药物敏感性相关，为靶向治疗提供潜在生物标志物。
---
**注意**：Chk2的Ser379磷酸化位点研究相对较少，若文献检索困难，建议进一步确认位点命名(如是否应为Thr68或Ser516等常见位点)，或查阅抗体供应商(如CST、Abcam)提供的技术文档及引用文献。
The Chk2 (Phospho-Ser379) antibody is a critical tool for studying the DNA damage response (DDR) pathway. Checkpoint kinase 2 (Chk2) is a serine/threonine kinase activated in response to DNA double-strand breaks or replication stress. Its activation involves phosphorylation at multiple sites, including Ser379 (human) or Ser380 (mouse), which is mediated by upstream kinases like ATM/ATR. This phosphorylation event stabilizes Chk2. promoting its dimer-to-monomer transition and subsequent kinase activity. Activated Chk2 phosphorylates downstream targets (e.g., p53. Cdc25) to regulate cell cycle arrest, DNA repair, or apoptosis.
The Chk2 (Phospho-Ser379) antibody specifically detects Chk2 phosphorylated at Ser379. serving as a marker for Chk2 activation in response to genotoxic stress. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study DDR mechanisms in cancer biology, chemotherapy response, and genomic stability research. Validation often includes testing in DNA-damaging agent-treated cells (e.g., etoposide, ionizing radiation) and verifying signal loss upon Chk2 knockdown or phosphatase treatment. Researchers should confirm species reactivity (commonly human/mouse) and optimize conditions to minimize cross-reactivity with similar phospho-epitopes. This antibody has been instrumental in elucidating Chk2's role in tumor suppression and therapeutic resistance.
×
