| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GFAP |
| Entrez GeneID | 2670; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human GFAP (Phospho-Ser13) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Phosphorylation-dependent antibody localization to GFAP Ser13 in astrocytic injury"**
*Authors: Tanaka K, et al.*
摘要：该研究验证了Phospho-Ser13 GFAP抗体的特异性，证明其在脑损伤模型中能选择性标记活化的星形胶质细胞，并发现磷酸化水平与损伤严重程度相关。
2. **"GFAP phosphorylation at Ser13 regulates filament dynamics and glioma cell motility"**
*Authors: Chen L, et al.*
摘要：通过Phospho-Ser13抗体，研究发现胶质瘤细胞中GFAP的磷酸化调控细胞骨架重组，影响肿瘤细胞迁移，为靶向治疗提供新见解。
3. **"A novel anti-phospho-GFAP (Ser13) antibody reveals phosphorylation in Alzheimer's disease pathology"**
*Authors: Smith J, et al.*
摘要：开发并验证了一种高特异性抗体，用于检测阿尔茨海默病患者脑组织中Ser13磷酸化GFAP，揭示其与淀粉样斑块周围胶质增生的关联。
4. **"Dynamic phosphorylation of GFAP Ser13 modulates astrocyte morphology during reactive gliosis"**
*Authors: Park S, et al.*
摘要：利用Phospho-Ser13抗体，研究显示缺血性脑损伤后GFAP的磷酸化动态调节星形胶质细胞形态变化，影响胶质瘢痕形成。
*注：以上文献信息为示例性质，实际引用需根据具体研究核实准确性。建议通过PubMed或Google Scholar以关键词“GFAP Ser13 phosphorylation antibody”检索最新文献。*
GFAP (Glial Fibrillary Acidic Protein) is a key intermediate filament protein predominantly expressed in astrocytes, playing a critical role in maintaining structural integrity and modulating cell motility in the central nervous system. Phosphorylation of GFAP at specific serine residues, such as Ser13. regulates its assembly-disassembly dynamics, impacting astrocyte morphology and function during physiological processes or pathological states. The GFAP (Phospho-Ser13) antibody is a specialized tool designed to detect GFAP when phosphorylated at serine 13. a post-translational modification associated with astrocyte activation, reactive gliosis, and cellular stress responses.
This antibody is widely used in neuroscience research to investigate astroglial responses in neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), traumatic brain injury, and gliomas. Phosphorylation at Ser13 is linked to cell cycle progression (e.g., G2/M phase) and cytoskeletal remodeling, making the antibody valuable for studying mechanisms underlying astrocyte reactivity or proliferation. Validation typically involves techniques like Western blotting, immunohistochemistry, or immunofluorescence, often using brain tissue or cultured astrocytes under conditions inducing phosphorylation (e.g., oxidative stress, injury models).
Its specificity for the phosphorylated epitope allows researchers to distinguish activated astrocytes from quiescent ones, offering insights into disease mechanisms or therapeutic targets. However, optimal application requires careful optimization to avoid cross-reactivity with non-phosphorylated GFAP or similar epitopes in related proteins.
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