| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CBX1, CBX, Chromobox protein homolog 1, HP1 beta, HP1Hs-beta, Heterochromatin protein 1-beta, HP1Hsbeta, M31, Modifier 1 protein, MOD1, HP1 beta homolog, HP1-BETA, Chromobox homolog 1, Heterochromatin protein p25, p25beta |
| Entrez GeneID | 10951; |
| WB Predicted band size | 25kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human HP1-β (Phospho-Thr51) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是基于HP1-β (Phospho-Thr51)相关研究的模拟参考文献示例(非真实文献，仅供格式参考)：
1. **"Phosphorylation of HP1β at Thr51 Modulates Heterochromatin Assembly in Response to DNA Damage"**
- **作者**: Lee S. et al.
- **摘要**: 本研究揭示了DNA损伤条件下HP1-β在Thr51位点的磷酸化如何影响其与异染色质蛋白(如SUV39H1)的相互作用，从而调控染色质凝缩和DNA修复效率。
2. **"Dynamic Phosphorylation of HP1β Regulates Mitotic Chromosome Segregation"**
- **作者**: Gupta R. & Patel A.
- **摘要**: 通过细胞周期分析，本文证明Thr51磷酸化在HP1-β的纺锤体附着和染色体分离中起关键作用，磷酸化缺失导致有丝分裂错误和基因组不稳定性。
3. **"HP1β Phospho-Thr51 as an Epigenetic Marker for Active Transcription Sites"**
- **作者**: Müller J. et al.
- **摘要**: 研究发现HP1-β的Thr51磷酸化状态与基因转录活性相关，磷酸化形式在染色质开放区域富集，提示其可能参与转录激活而非传统沉默功能。
4. **"A Kinase-Screen Identifies CDK2 as the Primary Kinase Targeting HP1β at Thr51"**
- **作者**: Chen X. et al.
- **摘要**: 通过激酶筛选和体外实验，本文鉴定了CDK2为HP1-β Thr51位点的关键磷酸化激酶，并验证了该修饰在细胞周期G1/S转换中的调控作用。
(注：以上为模拟内容，实际文献需通过PubMed等数据库检索确认。)
The HP1-β (Phospho-Thr51) antibody is a specialized tool used to study post-translational modifications of Heterochromatin Protein 1-beta (HP1-β), a key regulator of chromatin structure and gene silencing. HP1-β, encoded by the *Cbx1* gene, is a conserved component of heterochromatin that facilitates chromatin compaction and transcriptional repression by binding to methylated histone H3 lysine 9 (H3K9me3) via its chromodomain. Phosphorylation at threonine 51 (Thr51) within the hinge region of HP1-β is a dynamic regulatory mechanism influencing its molecular interactions and subcellular localization. This modification is implicated in modulating HP1-β’s ability to associate with chromatin and other proteins, thereby affecting heterochromatin stability, DNA repair, and epigenetic regulation.
The HP1-β (Phospho-Thr51) antibody specifically recognizes the phosphorylated form of Thr51. enabling researchers to investigate the spatial-temporal dynamics of this modification under various cellular conditions, such as cell cycle progression, stress responses, or differentiation. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore HP1-β’s role in maintaining genomic integrity, telomere function, and gene expression control. Dysregulation of HP1-β or its phosphorylation has been linked to diseases including cancer and aging-related disorders, making this antibody a valuable tool for both basic research and translational studies in epigenetics and chromatin biology.
×
