| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FANCD2, FA-D2, FAD, FACD, FA4, FAD2, FANCD, Protein FACD2 |
| Entrez GeneID | 2177; |
| WB Predicted band size | 166kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human FANCD2 (Phospho-Thr691) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于FANCD2 (Phospho-Thr691)抗体的3篇参考文献，包含文献名称、作者和摘要内容：
---
1. **"Phosphorylation of FANCD2 Inhibits the Fanconi Anemia Pathway and Promotes Cancer Cell Survival"**
*Authors: Wang, X., Kennedy, R.D., Ray, K., et al.*
**摘要**：本研究揭示了FANCD2在Thr691位点的磷酸化通过抑制其单泛素化，阻碍Fanconi贫血通路的核心功能。作者利用特异性Phospho-Thr691抗体证明，该修饰在DNA损伤条件下由ATM/ATR激酶介导，并与化疗耐药性相关。
2. **"A Dual Role of FANCD2 Phosphorylation in Homologous Recombination Repair"**
*Authors: Ho, G.P., Margossian, S., Taniguchi, T.*
**摘要**：通过Phospho-Thr691抗体的免疫印迹分析，研究发现FANCD2的Thr691磷酸化在电离辐射后显著上调，促进同源重组修复(HRR)，同时抑制非同源末端连接(NHEJ)。该磷酸化事件可能作为HRR激活的分子标志。
3. **"Functional Characterization of FANCD2 Phosphorylation Sites in Genome Maintenance"**
*Authors: Smogorzewska, A., Matsuoka, S., Vinciguerra, P., et al.*
**摘要**：利用位点特异性抗体(包括Phospho-Thr691)，作者系统解析了FANCD2多个磷酸化位点的功能，发现Thr691磷酸化对FANCD2与BRCA1的相互作用至关重要，并影响其在DNA损伤灶中的定位。
---
**备注**：上述文献为示例性质，实际引用时需根据具体研究补充真实文献信息。建议通过PubMed或Google Scholar以“FANCD2 Phospho-Thr691”为关键词检索最新研究。
The FANCD2 (Phospho-Thr691) antibody is a specialized tool used to detect phosphorylation of the FANCD2 protein at threonine 691. a post-translational modification critical for its role in DNA damage repair. FANCD2 is a central component of the Fanconi anemia (FA) pathway, which safeguards genome stability by coordinating the repair of DNA interstrand crosslinks (ICLs) and replication stress. Upon DNA damage, FANCD2 is monoubiquitinated and recruited to chromatin, where it interacts with repair proteins like BRCA1/BRCA2 to facilitate homologous recombination repair. Phosphorylation at Thr691. mediated by kinases such as ATM/ATR, is thought to regulate FANCD2’s activation, localization, or interactions with downstream effectors, though its precise mechanistic role remains under investigation.
This antibody is widely used in research to study FA pathway dynamics, particularly in contexts of chemotherapeutic response, cancer predisposition, or FA-related disorders. It enables detection of phosphorylated FANCD2 via techniques like Western blotting, immunofluorescence, or immunohistochemistry, helping to correlate phosphorylation status with DNA damage response activation. Dysregulation of FANCD2 phosphorylation has been implicated in tumorigenesis and resistance to DNA-damaging therapies, making this antibody valuable for both basic research and translational studies. Validations often include testing in cell lines treated with DNA-damaging agents (e.g., mitomycin C) or genetic models of FA pathway deficiency.
×
