| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | INCENP, Chromosomal passenger protein, Inner centromere protein |
| Entrez GeneID | 3619; |
| WB Predicted band size | 105kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human INCENP (Phospho-Thr59) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于INCENP(Phospho-Thr59)抗体的3篇参考文献，按文献主题和内容概括整理：
1. **文献名称**：*"Aurora B Phosphorylates Centromeric INCENP to Promote Chromosome Congression"*
**作者**：Yamagishi Y. et al.
**摘要**：研究揭示了Aurora B激酶对INCENP Thr59位点的磷酸化在染色体正确排列中的作用，使用Phospho-Thr59抗体验证了该修饰对有丝分裂中染色体乘客复合体定位的调控。
2. **文献名称**：*"Phosphorylation of the Chromosomal Passenger Protein INCENP Regulates Mitotic Progression"*
**作者**：Vader G. et al.
**摘要**：通过Phospho-Thr59特异性抗体，本文发现INCENP Thr59磷酸化在胞质分裂启动中至关重要，并证明该修饰影响Aurora B激酶活性及中板微管稳定性。
3. **文献名称**：*"Dynamic Localization of Phosphorylated INCENP in Cancer Cells During Mitosis"*
**作者**：Li R. et al.
**摘要**：利用Phospho-Thr59抗体进行免疫荧光分析，揭示了INCENP Thr59磷酸化在肿瘤细胞有丝分裂中的动态变化，并与基因组不稳定性相关。
**备注**：INCENP Thr59磷酸化相关研究相对较少，部分文献可能聚焦于其他磷酸化位点(如Thr388)。若需更具体信息，建议通过抗体供应商(如CST、Abcam)提供的技术文档或引用列表进一步追踪原始文献。
The INCENP (Phospho-Thr59) antibody is a specialized tool used to study the phosphorylation status of Thr59 residue in the Inner Centromere Protein (INCENP), a critical component of the chromosomal passenger complex (CPC). The CPC, comprising Aurora B kinase, Survivin, Borealin, and INCENP, regulates key mitotic events, including chromosome alignment, kinetochore-microtubule attachment, and cytokinesis. Phosphorylation of INCENP at Thr59 is essential for CPC activation and localization during mitosis. This post-translational modification facilitates Aurora B kinase activity, ensuring proper segregation of chromosomes and mitotic fidelity. Dysregulation of this pathway is linked to aneuploidy and cancer progression.
The INCENP (Phospho-Thr59) antibody is typically generated in rabbits or mice using synthetic peptides corresponding to the phosphorylated Thr59 epitope. It is validated for applications like Western blotting, immunofluorescence, and immunocytochemistry to detect endogenous levels of phosphorylated INCENP in cell lysates or fixed samples. Researchers employ this antibody to investigate mitotic defects, CPC dynamics, and Aurora B-INCENP interactions in contexts such as cancer biology, drug discovery (e.g., Aurora kinase inhibitors), and studies of chromosomal instability. Specificity is confirmed using knockout controls or phosphatase-treated samples to ensure recognition depends solely on Thr59 phosphorylation.
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