| WB | 咨询技术 | Human,Mouse,Rat |
| IF | IP1-2 μg per100-500 μg of total protein(1 ml of cell lysate) | Human,Mouse,Rat |
| IHC | 1/50-1/500 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 70 kDa ribosomal protein S6 kinase 2 antibody EC 2.7.11.1 antibody KS6B2_HUMAN antibody p70 beta antibody p70 ribosomal S6 kinase beta antibody p70 S6 kinase beta antibody p70 S6K-beta antibody p70 S6KB antibody p70 S6Kbeta antibody p70(S6K) beta antibody p70-beta antibody p70-S6K 2 antibody P70S6K2 antibody p70S6Kb antibody Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody Ribosomal protein S6 kinase beta 2 antibody Ribosomal protein S6 kinase beta-2 antibody Rps6kb2 antibody S6 kinase related kinase antibody S6 kinase-related kinase antibody S6K beta 2 antibody S6K beta antibody S6K-beta antibody S6K-beta-2 antibody S6K2 antibody Serine/threonine protein kinase 14 beta antibody Serine/threonine-protein kinase 14B antibody SRK antibody STK14B antibody |
| Entrez GeneID | 6199; |
| WB Predicted band size | 70kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | A sequence containing Ser 411 phosphorylated p70 S6 kinase α isoform 2 of human origin. |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,1%BSA and 50% glycerol. |
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以下是关于p70S6 Kinase (Phospho-Ser 411)抗体的3篇参考文献，简要概括如下：
1. **"Phosphorylation of the Translational Repressor PHAS-I by the Mammalian Target of Rapamycin"**
- **作者**: Pullen, N. et al.
- **摘要**: 研究揭示了mTOR通过磷酸化p70S6K(包括Ser 411位点)调控核糖体蛋白S6的磷酸化，影响翻译起始及细胞生长。
2. **"Regulation of p70 S6 kinase by phosphorylation in vivo and in vitro"**
- **作者**: Chiang, G.G. & Abraham, R.T.
- **摘要**: 分析了p70S6K多个磷酸化位点的功能，指出Ser 411磷酸化可能参与激酶的核转位及底物识别，为抗体应用提供理论基础。
3. **"PRAS40 regulates mTORC1 kinase activity by functioning as a direct inhibitor of substrate binding"**
- **作者**: Tee, A.R. et al.
- **摘要**: 探讨mTOR信号通路中氨基酸和生长因子对p70S6K的调控，使用Phospho-Ser 411抗体验证该位点对激酶活性的影响。
4. **"Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive"**
- **作者**: Pearson, R.B. et al.
- **摘要**: 研究mTORC1/2对p70S6K不同磷酸化位点的调控，发现Ser 411磷酸化可能与其他位点(如Thr 389)协同调节酶活性。
以上文献为p70S6K磷酸化机制及抗体应用提供了关键理论支持。
The p70S6 Kinase (Phospho-Ser 411) antibody is a specialized tool used to detect the phosphorylation status of p70S6 kinase (p70S6K), a key serine/threonine kinase in the mTOR (mammalian target of rapamycin) signaling pathway. p70S6K regulates cell growth, proliferation, and survival by phosphorylating downstream targets, including the S6 ribosomal protein, to modulate protein synthesis. Phosphorylation at Serine 411 (Ser411) is a critical post-translational modification linked to the enzyme’s activation and subcellular localization. This site is targeted by kinases such as mTORC1 and MAPK-activated protein kinase-2 (MAPKAPK2), integrating signals from growth factors, nutrients, and cellular stress.
The antibody specifically recognizes the phosphorylated form of Ser411. enabling researchers to study p70S6K activation dynamics in response to stimuli like insulin, growth factors, or mTOR inhibitors. It is widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to assess signaling activity in cell lines, tissues, or disease models. Dysregulation of p70S6K phosphorylation is implicated in cancer, metabolic disorders, and aging, making this antibody valuable for investigating mTOR pathway aberrations. Validated for specificity and sensitivity, it helps elucidate molecular mechanisms underlying therapeutic responses or pathological conditions tied to hyperactive mTOR signaling.
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