| WB | 咨询技术 | Human,Mouse,Rat |
| IF | IP1-2 μg per100-500 μg of total protein (1 ml of cell lysate) | Human,Mouse,Rat |
| IHC | 1/50-500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; TCF-14; HNF4alpha |
| Entrez GeneID | 3172 |
| clone | 8D12E3 |
| WB Predicted band size | 53 kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HNF4A (AA: 1-150) expressed in HEK293-6e cells supernatant. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PKR (Phospho-Thr446)抗体的参考文献示例(注：以下内容为假设性示例，具体文献可能需要通过学术数据库验证)：
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1. **文献名称**: "Phosphorylation of PKR at Thr446 is critical for its activation in response to viral infection"
**作者**: Smith A, et al.
**摘要**: 本研究揭示了PKR在病毒感染过程中通过Thr446位点的磷酸化被激活的机制。作者利用Phospho-Thr446特异性抗体，证实了该位点的磷酸化与PKR激酶活性的直接关联，并发现其对抗病毒免疫应答至关重要。
2. **文献名称**: "Role of PKR Thr446 phosphorylation in Alzheimer's disease pathology"
**作者**: Lee B, et al.
**摘要**: 通过使用Phospho-Thr446抗体，研究发现阿尔茨海默病模型中PKR的异常磷酸化与tau蛋白过度磷酸化相关，提示Thr446位点可能成为神经退行性疾病的潜在治疗靶点。
3. **文献名称**: "PKR autophosphorylation at Thr446 regulates its interaction with eIF2α during cellular stress"
**作者**: Chen C, et al.
**摘要**: 该文献利用Phospho-Thr446抗体证明，内质网应激条件下PKR在Thr446位点的自磷酸化增强了其与eIF2α的结合能力，从而调控蛋白质翻译抑制和细胞凋亡信号通路。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词“PKR Thr446 phosphorylation antibody”获取最新研究。
The PKR (Phospho-Thr446) antibody is a specialized tool used to detect the activated form of Protein Kinase R (PKR), a key enzyme in cellular stress responses. PKR, also known as EIF2AK2. is a serine/threonine kinase induced by interferons and activated by double-stranded RNA (dsRNA) during viral infections or cellular stress. Its activation involves autophosphorylation at specific residues, including Thr446 and Thr451. which are critical for its enzymatic activity. The Phospho-Thr446 antibody specifically recognizes PKR when phosphorylated at Thr446. serving as a marker for its activation status.
This antibody is widely used in research to study PKR's role in innate immunity, antiviral defense, and stress signaling pathways. Upon activation, PKR phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF2α), halting global protein synthesis to limit viral replication while promoting stress granule formation. Dysregulation of PKR activity has been implicated in neurodegenerative diseases, cancer, and chronic inflammation.
Researchers employ the PKR (Phospho-Thr446) antibody in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess PKR activation in cell lines, tissues, or experimental models under conditions such as viral infection, endoplasmic reticulum stress, or cytokine stimulation. Proper controls, including total PKR detection and phosphatase-treated samples, are essential to validate specificity. Its application enhances understanding of cellular stress responses and potential therapeutic targets.
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