| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SK 2; SK-2; SPK 2; SPK-2 |
| Entrez GeneID | 56848; |
| WB Predicted band size | 69kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Fusion protein corresponding to residues near the C terminal of human sphingosine kinase 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SPHK2抗体的3篇示例参考文献，内容基于常见研究方向构建，建议通过学术数据库获取具体文献：
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1. **文献名称**：*Role of Sphingosine Kinase 2 in Macrophage Migration and Inflammatory Signaling*
**作者**：Smith A, et al.
**摘要**：研究利用特异性SPHK2抗体，通过Western blot和免疫荧光技术，揭示了SPHK2在巨噬细胞趋化性和炎症因子分泌中的调控作用，证实抗体在检测内源性蛋白中的高特异性。
2. **文献名称**：*Monoclonal Antibody Development for Human SPHK2 and Its Application in Cancer Diagnostics*
**作者**：Zhang L, et al.
**摘要**：开发了一种人源SPHK2单克隆抗体，验证其在ELISA、免疫组化中的有效性，并应用于乳腺癌组织分析，表明SPHK2高表达与患者生存率降低显著相关。
3. **文献名称**：*SPHK2 Modulates Hepatic Lipid Metabolism: Insights from Antibody-Based Knockout Validation*
**作者**：Johnson R, et al.
**摘要**：通过SPHK2特异性抗体检测基因敲除小鼠模型，证明SPHK2缺失通过调节S1P信号通路减轻肝脏脂质堆积，抗体在蛋白水平验证中表现可靠。
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**备注**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台以关键词“SPHK2 antibody”“sphingosine kinase 2”检索获取。
Sphingosine kinase 2 (SPHK2) is an enzyme that catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P), a bioactive lipid mediator involved in cellular processes such as proliferation, apoptosis, and inflammation. Unlike its isoform SPHK1. which is primarily cytosolic and associated with pro-survival signaling, SPHK2 localizes to intracellular compartments, including the nucleus and mitochondria, and has context-dependent roles in promoting cell death or survival. SPHK2 is implicated in various diseases, including cancer, metabolic disorders, and neurodegenerative conditions, making it a target for therapeutic intervention.
SPHK2 antibodies are critical tools for studying the enzyme's expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to quantify SPHK2 levels in tissues or cells, assess its subcellular distribution, and explore regulatory mechanisms. Some antibodies can distinguish SPHK2 from SPHK1 due to isoform-specific epitopes, enabling precise investigation of their distinct roles. Additionally, SPHK2 antibodies aid in validating the efficacy of SPHK2 inhibitors in preclinical models, supporting drug development. Challenges in antibody development include ensuring specificity, as SPHK2 shares structural homology with SPHK1. and accounting for post-translational modifications that may affect detection. Overall, these antibodies advance research into S1P signaling and its therapeutic potential.
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