| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PYHIN4 |
| Entrez GeneID | 9447; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein corresponding to a region derived from internal residues of human absent in melanoma 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于AIM2抗体的3篇代表性文献，涵盖其功能、机制及应用的简要总结：
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1. **文献名称**：*AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA*
**作者**：Burckstummer, T. 等(2009)
**摘要**：该研究首次证实AIM2通过识别胞质DNA直接激活炎症小体，诱导caspase-1依赖的IL-1β成熟和细胞焦亡。研究中使用AIM2抗体进行免疫印迹(Western blot)和免疫共沉淀(Co-IP)，验证了AIM2在炎症反应中的核心作用。
2. **文献名称**：*The AIM2 inflammasome is essential for host defense against cytosolic bacteria and DNA viruses*
**作者**：Fernandes-Alnemri, T. 等(2010)
**摘要**：本文揭示AIM2炎症小体在抵御胞内细菌(如李斯特菌)和DNA病毒中的关键功能。通过AIM2特异性抗体的免疫荧光(IF)分析，证实病原体感染后AIM2与ASC的共定位，驱动宿主免疫防御。
3. **文献名称**：*AIM2 suppresses colorectal cancer progression via sensor and effector functions*
**作者**：Wilson, J.E. 等(2015)
**摘要**：研究报道AIM2在结直肠癌中具有抑癌作用，其表达缺失与患者不良预后相关。利用AIM2抗体进行免疫组化(IHC)分析，发现AIM2通过调控炎症信号和基因组稳定性抑制肿瘤进展。
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这些文献展示了AIM2抗体在机制研究(如炎症小体组装)、病原体防御及肿瘤免疫等领域的关键应用。如需具体实验方法(如抗体货号)，建议进一步查阅原文方法部分。
**Background of AIM2 Antibody**
The Absent in Melanoma 2 (AIM2) antibody targets the AIM2 protein, a key cytosolic DNA sensor involved in innate immune responses. AIM2 belongs to the PYHIN protein family, characterized by a PYD (pyrin) domain and an HIN (hematopoietic interferon-inducible nuclear antigen) domain. It plays a critical role in detecting double-stranded DNA (dsDNA) from pathogens or damaged host cells, triggering inflammasome assembly. Upon activation, AIM2 recruits ASC (apoptosis-associated speck-like protein) and procaspase-1. leading to caspase-1 activation, which promotes the maturation and release of pro-inflammatory cytokines (e.g., IL-1β, IL-18) and induces pyroptosis, a form of inflammatory cell death.
AIM2 antibodies are widely used in research to study inflammasome signaling, DNA-driven immune responses, and diseases linked to AIM2 dysregulation, such as autoimmune disorders (e.g., lupus), infections, and cancers. In cancer, AIM2 exhibits dual roles—acting as a tumor suppressor by promoting apoptosis or as an oncogene in certain contexts by fueling chronic inflammation. These antibodies enable detection of AIM2 expression, localization, and interaction partners via techniques like Western blot, immunofluorescence, and immunohistochemistry.
Recent studies also explore AIM2's potential as a therapeutic target or biomarker, emphasizing its relevance in immunology and precision medicine.
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