| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARHGEF5 |
| Uniprot No | Q12774 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-519aa |
| 氨基酸序列 | MGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSASPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA |
| 分子量 | 82.83 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**：*ARHGEF5 promotes oncogenic SRC-induced integrin recycling and cell migration*
**作者**：Zhang Y 等人
**摘要**：该研究揭示了ARHGEF5通过激活RhoA信号通路，增强SRC驱动的整合素循环，促进肿瘤细胞迁移和转移，尤其在乳腺癌中显示高表达并与不良预后相关。
2. **文献名称**：*Functional characterization of ARHGEF5 as a RhoA-specific guanine nucleotide exchange factor*
**作者**：Abe H 等人
**摘要**：首次报道ARHGEF5作为RhoA特异性GEF，通过其DH/PH结构域催化GDP-GTP交换，调控细胞骨架重塑及上皮细胞极性，为理解Rho GTP酶调控网络提供机制基础。
3. **文献名称**：*ARHGEF5 activates STAT3 via RhoA and drives colorectal cancer progression*
**作者**：Li C 等人
**摘要**：提出ARHGEF5通过激活STAT3信号通路促进结直肠癌细胞增殖和侵袭，靶向ARHGEF5可抑制小鼠模型中肿瘤生长，提示其作为潜在治疗靶点。
4. **文献名称**：*Structural basis of ARHGEF5-RhoA interaction and small molecule inhibition*
**作者**：Kim M 等人
**摘要**：解析ARHGEF5与RhoA复合物的晶体结构，鉴定关键结合界面，并筛选出抑制ARHGEF5-RhoA互作的小分子化合物，为开发抗癌药物提供结构依据。
注：以上文献信息为综合领域知识生成的示例，实际引用时请核对真实数据库及原文准确性。
ARHGEF5 (Adenylate Rho Guanine Nucleotide Exchange Factor 5), also known as TIM1 or ephexin-1. is a member of the Dbl family of Rho guanine nucleotide exchange factors (GEFs). These proteins activate Rho GTPases by catalyzing the exchange of GDP for GTP, enabling their participation in diverse cellular processes. Structurally, ARHGEF5 contains conserved DH (Dbl homology) and PH (Pleckstrin homology) domains critical for its GEF activity, as well as a transmembrane region that facilitates localization to the plasma membrane. It primarily activates RhoA and Rac1. key regulators of cytoskeletal dynamics, cell motility, and polarity.
ARHGEF5 is implicated in physiological and pathological processes, including embryonic development, angiogenesis, and cancer. Studies highlight its overexpression in malignancies like breast, lung, and gastric cancers, where it promotes tumor progression, invasion, and metastasis by enhancing epithelial-mesenchymal transition (EMT) and matrix degradation. Additionally, ARHGEF5 interacts with signaling pathways involving growth factor receptors (e.g., EGFR) and integrins, linking extracellular cues to intracellular cytoskeletal reorganization.
Recombinant ARHGEF5 is widely used to investigate its biochemical functions, regulatory mechanisms (e.g., phosphorylation, protein interactions), and therapeutic potential. Its role in cancer metastasis and resistance to chemotherapy underscores its appeal as a biomarker or target for anticancer strategies. However, its dual roles in tissue homeostasis versus disease contexts necessitate further exploration to harness its clinical relevance safely.
×
