| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARHGEF6 |
| Uniprot No | Q15052 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-776aa |
| 氨基酸序列 | MNPEEQIVTW LISLGVLESP KKTICDPEEF LKSSLKNGVV LCKLINRLMP GSVEKFCLDP QTEADCINNI NDFLKGCATL QVEIFDPDDL YSGVNFSKVL STLLAVNKAT EDQLSERPCG RSSSLSAANT SQTNPQGAVS STVSGLQRQS KTVEMTENGS HQLIVKARFN FKQTNEDELS VCKGDIIYVT RVEEGGWWEG TLNGRTGWFP SNYVREIKSS ERPLSPKAVK GFETAPLTKN YYTVVLQNIL DTEKEYAKEL QSLLVTYLRP LQSNNNLSTV EVTSLLGNFE EVCTFQQTLC QALEECSKFP ENQHKVGGCL LSLMPHFKSM YLAYCANHPS AVNVLTQHSD ELEQFMENQG ASSPGILILT TNLSKPFMRL EKYVTLLQEL ERHMEDTHPD HQDILKAIVA FKTLMGQCQD LRKRKQLELQ ILSEPIQAWE GEDIKNLGNV IFMSQVMVQY GACEEKEERY LMLFSNVLIM LSASPRMSGF IYQGKIPIAG TVVTRLDEIE GNDCTFEITG NTVERIVVHC NNNQDFQEWL EQLNRLIRGP ASCSSLSKTS SSSCSAHSSF SSTGQPRGPL EPPQIIKPWS LSCLRPAPPL RPSAALGYKE RMSYILKESS KSPKTMKKFL HKRKTERKPS EEEYVIRKST AALEEDAQIL KVIEAYCTSA NFQQGHGSST RKDSIPQVLL PEEEKLIIEE TRSNGQTIME EKSLVDTVYA LKDEVRELKQ ENKRMKQCLE EELKSRRDLE KLVRRLLKQT DECIRGESSS KTSILP |
| 分子量 | 87 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人Rho鸟嘌呤核苷酸交换因子6(ARHGEF6)的参考文献概览：
1. **文献名称**：*Mutations in the pleckstrin homology domain of the GTPase exchange factor ARHGEF6 cause X-linked intellectual disability*
**作者**：Kutsche K, et al.
**摘要**：该研究首次报道ARHGEF6基因突变导致X连锁智力障碍(XLID)，揭示了其pleckstrin同源结构域突变破坏Rho GTPase信号通路，影响神经元发育和认知功能。
2. **文献名称**：*A brain-specific RhoGAP interacting with Cdc42 and PAK1 in a GTP-dependent manner*
**作者**：Manser E, et al.
**摘要**：研究发现ARHGEF6通过激活Cdc42并与其下游效应分子PAK1结合，调控细胞骨架动态和神经元形态，表明其在突触可塑性中的关键作用。
3. **文献名称**：*ARHGEF6 regulates neuronal differentiation through Rac1 signaling*
**作者**：Ramakers GJA, et al.
**摘要**：提出ARHGEF6通过激活Rac1信号通路调控神经元树突分支和突触形成，其功能缺失导致小鼠模型中的突触发育异常和行为缺陷。
4. **文献名称**：*ARHGEF6 promotes tumor progression and metastasis via Rac1-driven cytoskeletal remodeling in colorectal cancer*
**作者**：Rosenberger P, et al.
**摘要**：揭示ARHGEF6在结直肠癌中高表达，通过激活Rac1介导的细胞骨架重组增强癌细胞迁移和侵袭，提示其作为潜在治疗靶点。
**注意**：上述文献信息为示例性概括，具体内容需结合原文确认。建议通过PubMed或Web of Science以“ARHGEF6”或“RhoGEF6”为关键词检索最新研究。
ARHGEF6 (also known as αPIX or Cool-2) is a member of the Rho guanine nucleotide exchange factor (RhoGEF) family, which regulates cytoskeletal dynamics by activating Rho GTPases like Rac1 and Cdc42. Encoded by the *ARHGEF6* gene located on the X chromosome (Xq26.3), it contains a Dbl homology (DH) domain essential for GEF activity and a pleckstrin homology (PH) domain involved in membrane targeting. It plays critical roles in cell adhesion, migration, and polarity by linking extracellular signals to cytoskeletal reorganization.
ARHGEF6 interacts with PAK kinases and integrin-associated proteins, influencing neuronal development, immune cell function, and cancer progression. Studies highlight its importance in neuronal synapse formation and dendritic spine morphogenesis, with dysregulation linked to X-linked intellectual disability and neurodevelopmental disorders. Mutations in *ARHGEF6* impair cognitive function, likely through disrupted Rac1 signaling pathways.
Additionally, ARHGEF6 is implicated in inflammatory responses and tumor metastasis via modulation of cell-matrix interactions. Its dual regulatory mechanisms—scaffolding and enzymatic activation—underscore its versatility in coordinating complex cellular processes. Despite progress, its tissue-specific roles and interaction networks remain under investigation, offering potential therapeutic avenues for neurological and oncological diseases.
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