| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ERCC2 |
| Uniprot No | P18074 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-760aa |
| 氨基酸序列 | MKLNVDGLLV YFPYDYIYPE QFSYMRELKR TLDAKGHGVL EMPSGTGKTV SLLALIMAYQ RAYPLEVTKL IYCSRTVPEI EKVIEELRKL LNFYEKQEGE KLPFLGLALS SRKNLCIHPE VTPLRFGKDV DGKCHSLTAS YVRAQYQHDT SLPHCRFYEE FDAHGREVPL PAGIYNLDDL KALGRRQGWC PYFLARYSIL HANVVVYSYH YLLDPKIADL VSKELARKAV VVFDEAHNID NVCIDSMSVN LTRRTLDRCQ GNLETLQKTV LRIKETDEQR LRDEYRRLVE GLREASAARE TDAHLANPVL PDEVLQEAVP GSIRTAEHFL GFLRRLLEYV KWRLRVQHVV QESPPAFLSG LAQRVCIQRK PLRFCAERLR SLLHTLEITD LADFSPLTLL ANFATLVSTY AKGFTIIIEP FDDRTPTIAN PILHFSCMDA SLAIKPVFER FQSVIITSGT LSPLDIYPKI LDFHPVTMAT FTMTLARVCL CPMIIGRGND QVAISSKFET REDIAVIRNY GNLLLEMSAV VPDGIVAFFT SYQYMESTVA SWYEQGILEN IQRNKLLFIE TQDGAETSVA LEKYQEACEN GRGAILLSVA RGKVSEGIDF VHHYGRAVIM FGVPYVYTQS RILKARLEYL RDQFQIREND FLTFDAMRHA AQCVGRAIRG KTDYGLMVFA DKRFARGDKR GKLPRWIQEH LTDANLNLTV DEGVQVAKYF LRQMAQPFHR EDQLGLSLLS LEQLESEETL KRIEQIAQQL |
| 分子量 | 86.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人ERCC2蛋白的3篇文献摘要示例：
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1. **"Expression and functional analysis of recombinant human ERCC2/XPD protein"**
*作者：Weber CA, et al.*
**摘要**：本文报道了重组人ERCC2蛋白在大肠杆菌中的表达与纯化方法，并验证其作为DNA解旋酶的活性。实验表明，重组ERCC2在核苷酸切除修复(NER)中依赖ATP水解，为研究DNA修复机制提供了工具。
2. **"Structural insights into the role of ERCC2 in transcription-coupled repair"**
*作者：Dubaele S, et al.*
**摘要**：通过X射线晶体学解析ERCC2与TFIIH复合体的结构，揭示其通过解旋酶结构域参与DNA损伤识别，解释了部分癌症相关突变影响修复功能的分子机制。
3. **"ERCC2 mutations disrupt ATPase activity in human trichothiodystrophy models"**
*作者：Kraemer KH, et al.*
**摘要**：利用重组ERCC2突变体，研究毛发硫营养不良(TTD)患者中ERCC2功能缺失对NER的影响，发现ATP结合位点突变导致修复缺陷，强调了其酶活性在遗传疾病中的作用。
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*注：以上文献为示例，实际引用需根据具体研究选择并核实准确性。*
Recombinant human ERCC2 protein, also known as XPD (xeroderma pigmentosum complementation group D), is a critical DNA repair enzyme encoded by the ERCC2 gene. As a member of the helicase family, it plays a dual role in transcription initiation and nucleotide excision repair (NER), a pathway that removes bulky DNA lesions caused by UV radiation, chemical agents, or oxidative stress. ERCC2/XPD functions as an ATP-dependent 5’→3’ DNA helicase within the multi-subunit TFIIH complex, unwinding DNA around damage sites during NER and facilitating transcriptional regulation by RNA polymerase II.
Mutations in ERCC2 are linked to xeroderma pigmentosum (XP), a genetic disorder characterized by extreme UV sensitivity and predisposition to skin cancer, as well as Cockayne syndrome and trichothiodystrophy, which manifest in developmental defects and premature aging. Recombinant ERCC2 protein, typically produced in bacterial or eukaryotic expression systems, enables structural and functional studies to dissect its roles in DNA repair mechanisms, helicase activity, and disease pathogenesis. Its applications extend to screening potential therapeutic agents targeting DNA repair deficiencies in cancers or exploring gene therapy strategies. The protein’s conserved structure and interaction partners across species also make it a valuable model for understanding genome stability maintenance.
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