| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ERF |
| Uniprot No | P50548 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-548aa |
| 氨基酸序列 | MKTPADTGFAFPDWAYKPESSPGSRQIQLWHFILELLRKEEYQGVIAWQGDYGEFVIKDPDEVARLWGVRKCKPQMNYDKLSRALRYYYNKRILHKTKGKRFTYKFNFNKLVLVNYPFIDVGLAGGAVPQSAPPVPSGGSHFRFPPSTPSEVLSPTEDPRSPPACSSSSSSLFSAVVARRLGRGSVSDCSDGTSELEEPLGEDPRARPPGPPDLGAFRGPPLARLPHDPGVFRVYPRPRGGPEPLSPFPVSPLAGPGSLLPPQLSPALPMTPTHLAYTPSPTLSPMYPSGGGGPSGSGGGSHFSFSPEDMKRYLQAHTQSVYNYHLSPRAFLHYPGLVVPQPQRPDKCPLPPMAPETPPVPSSASSSSSSSSSPFKFKLQPPPLGRRQRAAGEKAVAGADKSGGSAGGLAEGAGALAPPPPPPQIKVEPISEGESEEVEVTDISDEDEEDGEVFKTPRAPPAPPKPEPGEAPGASQCMPLKLRFKRRWSEDCRLEGGGGPAGGFEDEGEDKKVRGEGPGEAGGPLTPRRVSSDLQHATAQLSLEHRDS |
| 分子量 | 85.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ERF蛋白的3篇参考文献示例(注：文献为示例性内容，非真实存在)：
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**1. 文献名称**：*"Efficient expression and purification of recombinant human ERF protein in E. coli for functional studies"*
**作者**：Zhang L, et al.
**摘要**：本研究通过优化密码子和表达条件，在大肠杆菌中高效表达可溶性重组人ERF蛋白，并利用镍柱亲和层析纯化。纯化后的蛋白通过凝胶电泳和Western blot验证，证实其具有与天然ERF相似的DNA结合活性，为后续功能研究奠定基础。
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**2. 文献名称**：*"ERF regulates apoptosis in breast cancer cells via modulation of the PI3K/AKT pathway"*
**作者**：Chen R, et al.
**摘要**：通过体外实验发现，重组人ERF蛋白可抑制乳腺癌细胞增殖并诱导凋亡。机制研究表明，ERF通过下调PI3K/AKT信号通路的关键蛋白磷酸化水平发挥抑癌作用，提示其作为潜在治疗靶点的价值。
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**3. 文献名称**：*"Structural basis of ERF protein interaction with DNA: Insights from X-ray crystallography"*
**作者**：Kimura T, et al.
**摘要**：本研究解析了重组人ERF蛋白与靶DNA复合物的晶体结构(分辨率2.1 Å)，揭示了ERF的ETS结构域如何特异性识别DNA序列，并阐明了关键氨基酸残基在结合中的功能，为设计ERF相关抑制剂提供结构基础。
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如需更多领域细分文献(如疾病模型或药物开发)，可进一步调整研究背景和结论。
**Background of Recombinant Human ERF Protein**
The recombinant human ERF (ETS2 Repressor Factor) protein is a genetically engineered version of the native ERF protein, a member of the ETS transcription factor family. ERF regulates gene expression by binding to specific DNA sequences, playing critical roles in cellular processes such as proliferation, differentiation, apoptosis, and development. Structurally, ERF contains a conserved ETS domain responsible for DNA binding and a transcriptional repression domain, enabling it to modulate target gene activity.
Native ERF is implicated in various physiological and pathological conditions, including cancer, cardiovascular diseases, and developmental disorders. Dysregulation of ERF expression or function is associated with tumor progression, metastasis, and resistance to therapy, particularly in cancers like prostate, breast, and glioblastoma.
Recombinant ERF is typically produced using *E. coli* or mammalian expression systems, ensuring high purity and bioactivity for research applications. It serves as a vital tool for studying ERF-mediated signaling pathways, protein-DNA interactions, and its role as a tumor suppressor or context-dependent oncogene. Additionally, recombinant ERF aids in drug discovery, biomarker development, and functional assays to explore therapeutic targeting of ERF in diseases. Its use in *in vitro* and *in vivo* models helps clarify mechanisms underlying ERF's dual roles in cellular homeostasis and pathology.
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