| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ERGIC1 |
| Uniprot No | Q969X5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-290aa |
| 氨基酸序列 | MPFDFRRFDIYRKVPKDLTQPTYTGAIISICCCLFILFLFLSELTGFITTEVVNELYVDDPDKDSGGKIDVSLNISLPNLHCELVGLDIQDEMGRHEVGHIDNSMKIPLNNGAGCRFEGQFSINKVPGNFHVSTHSATAQPQNPDMTHVIHKLSFGDTLQVQNIHGAFNALGGADRLTSNPLASHDYILKIVPTVYEDKSGKQRYSYQYTVANKEYVAYSHTGRIIPAIWFRYDLSPITVKYTERRQPLYRFITTICAIIGGTFTVAGILDSCIFTASEAWKKIQLGKMH |
| 分子量 | 59 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ERGIC1蛋白的假设性参考文献，基于已知相关领域研究推测，实际文献需进一步验证：
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1. **文献名称**：*Molecular Cloning and Functional Characterization of Human ERGIC1 in Secretory Protein Trafficking*
**作者**：Zhang Y. et al.
**摘要**：研究报道了重组人ERGIC1蛋白的克隆与表达，通过哺乳动物细胞系统成功纯化该蛋白，并证明其在内质网-高尔基体间囊泡运输中协调COPII复合体的组装，影响分泌蛋白的定向转运。
2. **文献名称**：*Structural Insights into ERGIC1’s Role in Cargo Sorting via Cryo-EM Analysis*
**作者**：Smith A.L. & Kumar R.
**摘要**：利用重组人ERGIC1蛋白进行冷冻电镜结构解析，揭示其与Sec23/24复合体的互作界面，提出ERGIC1作为货物受体选择性招募特定分泌蛋白的分子机制。
3. **文献名称**：*ERGIC1 Dysregulation Promotes Tumor Metastasis: Studies with Recombinant Protein Models*
**作者**：Lee H. et al.
**摘要**：通过重组ERGIC1蛋白体外实验，发现其在乳腺癌细胞中高表达可通过激活MAPK通路促进侵袭转移，为癌症治疗提供潜在靶点。
4. **文献名称**：*Interaction Screening of ERGIC1 with ER Exit Site Components Using Recombinant Protein Pull-down Assays*
**作者**：Chen X. & Wang T.
**摘要**：基于重组ERGIC1的体外互作实验，鉴定其与Sar1 GTP酶及SNARE蛋白STX5的特异性结合，阐明其对早期分泌途径的调控网络。
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**注意事项**：
以上文献为示例性内容，实际研究中ERGIC1可能涉及更具体的功能或疾病模型。建议通过PubMed、Web of Science等数据库，以关键词"recombinant ERGIC1"或"ERGIC1 protein trafficking"查询最新文献，并核实准确性。
The endoplasmic reticulum-Golgi intermediate compartment 1 (ERGIC1) protein is a key component of the ERGIC, a dynamic membrane system involved in intracellular protein trafficking. It plays a critical role in the bidirectional transport of cargo between the endoplasmic reticulum (ER) and Golgi apparatus, facilitating secretory pathway function. ERGIC1 interacts with COPII and COPI vesicle coat proteins, aiding in vesicle formation, cargo sorting, and maintaining compartment integrity. Studies suggest its involvement in autophagy, lipid metabolism, and response to cellular stress, with potential links to diseases like cancer and neurodegenerative disorders. Recombinant human ERGIC1 protein, produced via heterologous expression systems (e.g., mammalian or insect cells), retains post-translational modifications crucial for its biological activity. It serves as a vital tool for studying ER-Golgi dynamics, membrane trafficking mechanisms, and disease-related pathways. Researchers use purified ERGIC1 in binding assays, structural studies, and drug screening, particularly for molecules targeting secretory pathway dysregulation. Its recombinant form enables detailed analysis of molecular interactions and regulatory networks in both physiological and pathological contexts.
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