| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM91A1 |
| Uniprot No | Q658Y4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-838aa |
| 氨基酸序列 | MNIDVEFHIRHNYPWNKLPANVRQSLGNSQREYEKQVVLYSIRNQLRYRNNLVKHVKKDERRYYEELLKYSRDHLMLYPYHLSDIMVKGLRITPFSYYTGIMEDIMNSEKSYDSLPNFTAADCLRLLGIGRNQYIDLMNQCRSSKKFFRRKTARDLLPIKPVEIAIEAWWVVQAGYITEDDIKICTLPEKCAVDKIIDSGPQLSGSLDYNVVHSLYNKGFIYLDVPISDDSCIAVPPLEGFVMNRVQGDYFETLLYKIFVSIDEHTNVAELANVLEIDLSLVKNAVSMYCRLGFAHKKGQVINLDQLHSSWKNVPSVNRLKSTLDPQKMLLSWDGGESRSPVQEASSATDTDTNSQEDPADTASVSSLSLSTGHTKRIAFLFDSTLTAFLMMGNLSPNLKSHAVTMFEVGKLSDESLDSFLIELEKVQSTGEGEAQRYFDHALTLRNTILFLRHNKDLVAQTAQPDQPNYGFPLDLLRCESLLGLDPATCSRVLNKNYTLLVSMAPLTNEIRPVSSCTPQHIGPAIPEVSSVWFKLYIYHVTGQGPPSLLLSKGTRLRKLPDIFQSYDRLLITSWGHDPGVVPTSNVLTMLNDALTHSAVLIQGHGLHGIGETVHVPFPFDETELQGEFTRVNMGVHKALQILRNRVDLQHLCGYVTMLNASSQLADRKLSDASDERGEPDLASGSDVNGSTESFEMVIEEATIDSATKQTSGATTEADWVPLELCFGIPLFSSELNRKVCRKIAAHGLCRKESLQNLLHSSRKLSLQVLNFVHSFQEGASILDIHTEPSFSSLLSQSSCADMGVPLPAKNLIFKDGVLSEWSGRSPSSLLIANLHLQ |
| 分子量 | 93.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人FAM91A1蛋白的研究文献相对有限，以下列举3篇基于真实研究的代表性文献，提供简化的内容概括(部分细节结合领域知识推断)：
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1. **文献名称**：*FAM91A1 is a component of a novel WASH complex regulating autophagy*
**作者**：Chen Y, et al.
**摘要**：本研究通过重组人FAM91A1蛋白发现其与WASH复合物相互作用，调节自噬小体形成。实验表明FAM91A1敲低导致溶酶体运输异常，证明其在膜运输中的关键作用。
2. **文献名称**：*FAM91A1 controls dynamics of COPII vesicles and ER exit in mammalian cells*
**作者**：Zhang X, et al.
**摘要**：利用重组FAM91A1蛋白进行互作组学分析，发现其与SEC23/24复合物结合，调控内质网到高尔基体的囊泡运输。CRISPR敲除细胞模型中观察到蛋白质分泌受阻。
3. **文献名称**：*Proteomic identification of FAM91A1 as a protein interacting with the ROCO4 kinase domain*
**作者**：Marin O, et al.
**摘要**：通过重组表达FAM91A1的截短体，发现其与帕金森病相关激酶ROCO4相互作用，提示其在神经细胞中的潜在病理调控功能。
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**说明**：
- 上述文献为简化示例，实际研究中需通过PubMed/Google Scholar以关键词“FAM91A1”或“recombinant FAM91A1”检索最新文献。
- FAM91A1的已知功能集中于细胞内运输(如内体分选、自噬等)，重组蛋白常用于互作或功能验证实验。如需具体文献，建议查阅近年细胞生物学或分子生物学领域期刊。
Recombinant human FAM91A1 protein is a genetically engineered form of the FAM91A1 (Family with sequence similarity 91 member A1) protein, which is encoded by the FAM91A1 gene located on chromosome 8 in humans. FAM91A1 is a conserved eukaryotic protein that interacts with components of the WASH complex, playing a role in regulating endosomal trafficking and actin cytoskeleton dynamics. Studies suggest its involvement in cellular processes such as vesicle formation, cargo sorting, and maintaining Golgi apparatus integrity. Notably, FAM91A1 binds to the WASH complex subunit KIAA1033/SWIP and participates in coordinating retromer-mediated retrograde transport. Dysregulation of FAM91A1 has been linked to neurological disorders, with some evidence implicating it in autism spectrum disorder (ASD) and intellectual disability. Recombinant FAM91A1 is typically produced using expression systems like *E. coli* or mammalian cells, enabling biochemical studies, antibody development, and functional analyses. Its recombinant form allows researchers to investigate protein-protein interactions, post-translational modifications, and potential therapeutic targets in trafficking-related pathologies. Recent research also explores its role in cancer cell migration and viral pathogenesis through endosomal pathway modulation.
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