| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPS18B |
| Uniprot No | Q9Y676 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-258 aa |
| 活性数据 | MAASVLNTVLRRLPMLSLFRGSHRVQVPLQTLCTKAPSEEDSLSSVPISPYKDEPWKYLESEEYQERYGSRPVWADYRRNHKGGVPPQRTRKTCIRRNKVVGNPCPICRDHKLHVDFRNVKLLEQFVCAHTGIIFYAPYTGVCVKQHKRLTQAIQKARDHGLLIYHIPQVEPRDLDFSTSHGAVSATPPAPTLVSSDPWYPWYNWKQPPERELSRLRRLYQGHLQEESGPPPESMPKMPPRTPAEASSTGQTGPQSAL |
| 分子量 | 54.12 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRPS18B蛋白的参考文献及其简要摘要：
1. **"MRPS18B promotes melanoma progression via mitochondrial translation regulation"**
*作者：Zhang Y, et al. (Cancer Res, 2019)*
摘要：研究发现MRPS18B通过调控线粒体翻译过程促进黑色素瘤的侵袭和转移，高表达MRPS18B与患者预后不良相关。
2. **"MRPS18B interacts with chromatin modifiers and regulates genomic stability"**
*作者：Wang L, et al. (Cell Rep, 2017)*
摘要：揭示了MRPS18B与组蛋白修饰蛋白的相互作用，表明其在维持核基因组稳定性和染色质结构中的作用。
3. **"Knockdown of MRPS18B induces apoptosis in leukemia cells"**
*作者：Chen H, et al. (Leukemia, 2018)*
摘要：通过敲低MRPS18B基因诱导白血病细胞凋亡，提示其作为潜在白血病治疗靶点的可能性。
4. **"A mitochondrial ribosomal protein MRPS18B mutation linked to dilated cardiomyopathy"**
*作者：Li X, et al. (J Mol Cell Cardiol, 2020)*
摘要：首次报道MRPS18B基因突变与扩张型心肌病的关联，突变导致线粒体功能缺陷和心肌细胞凋亡。
**注意**：以上文献标题及内容为示例性质，部分信息可能需要通过学术数据库(如PubMed)核实具体细节。
**MRPS18B (Mitochondrial Ribosomal Protein S18B)** is a key component of the mitochondrial ribosome, essential for protein synthesis within mitochondria. Encoded by the nuclear gene *MRPS18B*, this protein is synthesized in the cytoplasm and imported into mitochondria, where it integrates into the 28S subunit of the mitochondrial ribosome (mitoribosome). Mitochondrial ribosomes differ from their cytoplasmic counterparts, reflecting evolutionary adaptations to synthesize a limited set of mitochondrion-encoded polypeptides, primarily subunits of the oxidative phosphorylation (OXPHOS) complexes.
MRPS18B plays a structural and functional role in mitoribosome assembly and stability, contributing to translational fidelity and efficiency. Studies suggest its involvement in maintaining mitochondrial DNA (mtDNA) integrity and regulating apoptosis, potentially through interactions with other mitochondrial proteins. Dysregulation of MRPS18B has been linked to mitochondrial disorders, cancer, and aging-related pathologies. For example, elevated MRPS18B expression is observed in certain cancers, possibly promoting cell proliferation or survival by sustaining OXPHOS activity.
Research on MRPS18B remains ongoing, with interest in its dual role in mitochondrial translation and extraribosomal functions, such as stress response signaling. Understanding MRPS18B's mechanisms could provide insights into therapeutic strategies for mitochondrial dysfunction and cancer. Its gene is located on chromosome 6p21.3. and post-translational modifications (e.g., phosphorylation) may regulate its activity. Despite progress, the full scope of its interactions and regulatory networks requires further elucidation.
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