| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PASD1 |
| Uniprot No | Q8IV76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-773 aa |
| 活性数据 | MKMRGEKRRD KVNPKSSQRK LNWIPSFPTY DYFNQVTLQL LDGFMITLST DGVIICVAEN ISSLLGHLPA EIVGKKLLSL LPDEEKDEVY QKIILKFPLL NSETHIEFCC HLKRGNVEHG DSSAYENVKF IVNVRDICNE FPVVFSGLFS SHLCADFAAC VPQEDRLYLV GNVCILRTQL LQQLYTSKAV SDEAVLTQDS DEEPFVGELS SSQGQRGHTS MKAVYVEPAA AAAAAAISDD QIDIAEVEQY GPQENVHMFV DSDSTYCSST VFLDTMPESP ALSLQDFRGE PEVNPLYRAD PVDLEFSVDQ VDSVDQEGPM DQQDPENPVA PLDQAGLMDP VDPEDSVDLG AAGASAQPLQ PSSPVAYDII SQELELMKKL KEQLEERTWL LHDAIQNQQN ALELMMDHLQ KQPNTLRHVV IPDLQSSEAV PKKQQKQHAG QVKRPLPHPK DVKCFCGLSL SNSLKNTGEL QEPCVAFNQQ QLVQQEQHLK EQQRQLREQL QQLREQRKVQ KQKKMQEKKK LQEQKMQEKK KLQEQRRQKK KKLQERKKWQ GQMLQKEPEE EQQKQQLQEQ PLKHNVIVGN ERVQICLQNP RDVSVPLCNH PVRFLQAQPI VPVQRAAEQQ PSGFYQDENC GQQEDESQSF YPEAYQGPPV NQLPLIDTSN SEAISSSSIP QFPITSDSTI STLETPQDYI RLWQELSDSL GPVVQVNTWS CDEQGTLHGQ PTYHQVQVSE VGVEGPPDPQ AFQGPAAYQP DQMRSAEQTR LMPAEQRDSN KPC |
| 分子量 | 87.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PASD1蛋白的示例性参考文献(内容为概括性描述，实际引用需核实原文)：
1. **"Expression and purification of recombinant human PASD1 protein for cancer immunotherapy"**
*作者：Smith A, et al.*
*摘要*：研究报道了一种在大肠杆菌中高效表达并纯化重组人PASD1蛋白的方法，验证了其作为癌症-睾丸抗原的免疫原性，为基于PASD1的疫苗开发提供基础。
2. **"PASD1 as a novel biomarker in leukemia: diagnostic potential of recombinant protein-based assays"**
*作者：Lee B, et al.*
*摘要*：通过重组人PASD1蛋白开发ELISA检测方法，证明其在急性髓系白血病患者中高表达，提示其作为血液肿瘤诊断标志物的潜力。
3. **"Structural characterization of PASD1 protein and its role in melanoma progression"**
*作者：Chen C, et al.*
*摘要*：利用重组PASD1蛋白解析其三维结构，并通过体外实验证实其通过调控细胞周期相关通路促进黑色素瘤侵袭的分子机制。
4. **"Recombinant PASD1 protein triggers antigen-specific T cell responses in ovarian cancer"**
*作者：Gupta R, et al.*
*摘要*：实验表明重组PASD1蛋白可激活患者外周血T细胞产生特异性免疫反应，支持其作为卵巢癌过继性免疫治疗的靶标。
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**注意**：以上内容为根据PASD1蛋白已知生物学背景构建的示例，实际文献需通过PubMed、Web of Science等平台检索。建议结合关键词“PASD1 recombinant protein”或“PASD1 cancer-testis antigen”获取真实论文。
PASD1 (PAS domain-containing protein 1) is a cancer-testis antigen (CTA) predominantly expressed in germline cells and epigenetically silenced in most somatic tissues. Its aberrant reactivation is observed in various malignancies, particularly hematologic cancers like multiple myeloma, leukemia, and lymphoma. As a member of the PAS protein family, PASD1 contains a conserved PAS domain implicated in protein-protein interactions, ligand binding, or environmental sensing. Its oncogenic role remains under investigation but is linked to modulating transcriptional regulation, cell cycle progression, and immune evasion.
Recombinant human PASD1 protein is engineered for research and therapeutic applications. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it retains antigenic epitopes critical for immunogenicity studies. PASD1's restricted expression in normal tissues and tumor-specific reactivation make it a promising target for immunotherapy, including cancer vaccines and adoptive T-cell therapies. Current research focuses on characterizing its structure-function relationships, autoantigen potential, and utility in developing biomarkers or targeted treatments. However, challenges persist in understanding its precise molecular mechanisms and optimizing its clinical translation due to variable expression across tumors and potential immune tolerance.
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