| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAP30 |
| Uniprot No | O75446 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-220 aa |
| 活性数据 | MNGFTPDEMS RGGDAAAAVA AVVAAAAAAA SAGNGTGAGT GAEVPGAGAV SAAGPPGAAG PGPGQLCCLR EDGERCGRAA GNASFSKRIQ KSISQKKVKI ELDKSARHLY ICDYHKNLIQ SVRNRRKRKG SDDDGGDSPV QDIDTPEVDL YQLQVNTLRR YKRHFKLPTR PGLNKAQLVE IVGCHFRSIP VNEKDTLTYF IYSVKNDKNK SDLKVDSGVH |
| 分子量 | 23.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SAP30蛋白的3篇参考文献(均为虚构，仅作示例参考)：
1. **"SAP30 is a component of the Sin3/HDAC complex and mediates transcriptional repression"**
- 作者：Zhang, Y., et al.
- 摘要：研究首次证明了人源SAP30蛋白通过重组表达与Sin3及组蛋白去乙酰化酶(HDAC)形成复合物，在体外抑制靶基因转录活性，揭示了其在表观遗传调控中的作用。
2. **"Structural and functional analysis of recombinant human SAP30 in chromatin remodeling"**
- 作者：Kumar, R., et al.
- 摘要：通过大肠杆菌系统重组表达并纯化SAP30蛋白，发现其通过结合磷酸化组蛋白标志物参与染色质重构，为解析SAP30的分子机制提供了结构基础。
3. **"Recombinant SAP30 modulates HDAC activity in cancer cell lines"**
- 作者：Chen, L., et al.
- 摘要：利用昆虫细胞表达系统获得高纯度重组SAP30蛋白，证实其能增强HDAC复合物活性，抑制肿瘤细胞增殖，提示其作为潜在癌症治疗靶点。
(注：以上文献为示例，实际引用时请核实真实文献。)
SAP30 (Sin3A-associated protein 30) is a key component of the SIN3/histone deacetylase (HDAC) corepressor complex, which plays a critical role in epigenetic regulation and transcriptional repression. Discovered as a conserved nuclear protein, SAP30 interacts directly with Sin3A and HDAC1/2. facilitating chromatin remodeling and gene silencing by promoting histone deacetylation and subsequent chromatin condensation. It contains distinct functional domains, including an N-terminal zinc finger motif and a C-terminal region essential for protein-protein interactions, enabling its recruitment to specific genomic loci via transcription factors or DNA-binding adaptors.
Recombinant human SAP30 protein is widely used to study molecular mechanisms of gene repression, cellular differentiation, and cancer development. Dysregulation of SAP30-associated pathways has been linked to malignancies, neurological disorders, and viral latency. Researchers employ recombinant SAP30 in vitro to reconstitute SIN3/HDAC complexes, analyze enzymatic activities, and investigate its role in tumor suppressor networks. Its production through recombinant DNA technology ensures high purity and consistency for structural studies, protein interaction assays, and drug discovery targeting epigenetic modifiers. Understanding SAP30’s function provides insights into therapeutic strategies for diseases involving aberrant epigenetic regulation.
×
