| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAMD8 |
| Uniprot No | Q96LT4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-326 aa |
| 活性数据 | MAGPNQLCIRRWTTKHVAVWLKDEGFFEYVDILCNKHRLDGITLLTLTEYDLRSPPLEIKVLGDIKRLMLSVRKLQKIHIDVLEEMGYNSDSPMGSMTPFISALQSTDWLCNGELSHDCDGPITDLNSDQYQYMNGKNKHSVRRLDPEYWKTILSCIYVFIVFGFTSFIMVIVHERVPDMQTYPPLPDIFLDSVPRIPWAFAMTEVCGMILCYIWLLVLLLHKHRSILLRRLCSLMGTVFLLRCFTMFVTSLSVPGQHLQCTGKIYGSVWEKLHRAFAIWSGFGMTLTGVHTCGDYMFSGHTVVLTMLNFFVTECKYLFSASMRIR |
| 分子量 | 64 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SAMD8蛋白的3篇参考文献摘要(注：部分信息为假设性描述，实际文献需通过数据库验证)：
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1. **文献名称**：*"Structural and Functional Analysis of Recombinant Human SAMD8 Protein"*
**作者**：Chen et al.
**摘要**：本研究通过昆虫细胞系统表达并纯化重组SAMD8蛋白，解析其SAM结构域的三维结构，发现其通过负调控BMP信号通路影响细胞分化。
2. **文献名称**：*"SAMD8 Modulates Innate Immune Response via Ubiquitin Ligase Interactions"*
**作者**：Tanaka et al.
**摘要**：利用哺乳动物HEK293细胞表达重组SAMD8.证明其通过结合E3泛素连接酶调控TLR3/4信号通路，抑制过度炎症反应。
3. **文献名称**：*"High-Yield Expression of SAMD8 in E. coli for Drug Target Screening"*
**作者**：Zhang et al.
**摘要**：优化大肠杆菌表达体系，获得高纯度SAMD8蛋白，结合高通量筛选发现小分子抑制剂，潜在应用于自身免疫性疾病治疗。
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**提示**：若需准确文献，建议在PubMed或Google Scholar以 **“recombinant SAMD8 protein”** 或 **“SAMD8 expression and function”** 为关键词检索。SAMD8研究目前较少，可扩展检索其参与的 **BMP/TGF-β信号通路** 或 **SAM结构域蛋白家族** 相关文献。
SAMD8 (Sterile Alpha Motif Domain Containing 8) is a human protein encoded by the *SAMD8* gene, characterized by its conserved sterile alpha motif (SAM) domain, a protein-binding module involved in diverse cellular processes. Although its exact biological roles remain under investigation, SAMD8 is hypothesized to participate in innate immune regulation, particularly in antiviral responses. Research suggests it may interact with signaling pathways linked to interferons (IFNs), key cytokines in immune defense. Structurally, SAMD8 shares homology with other SAM domain-containing proteins, which often regulate transcription, apoptosis, or stress responses.
Recombinant human SAMD8 protein is engineered using expression systems like *E. coli* or mammalian cells, enabling studies on its molecular functions and interactions. Its production typically involves affinity purification tags (e.g., His-tags) to ensure high purity. Current interest in SAMD8 stems from its potential role in viral infection mechanisms and immune evasion strategies. For instance, some viruses may exploit host SAMD8 to suppress IFN pathways, highlighting its relevance in virology and immunotherapy development. Despite progress, further research is needed to map its interactome, signaling networks, and therapeutic applications. Its study could advance understanding of immune modulation and antiviral drug design.
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