| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEC31B |
| Uniprot No | Q9NQW1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-348 aa |
| 活性数据 | MKLKELERPAVQAWSPASQYPLYLATGTSAQQLDSSFSTNGTLEIFEVDFRDPSLDLKHRGVLSALSRFHKLVWGSFGSGLLESSGVIAGGGDNGMLILYNVTHILSSGKEPVIAQKQKHTGAVRALDFNPFQGNLLASGASDSEIFIWDLNNLNVPMTLGSKSQPPEDIKALSWNRQAQHILSSAHPSGKAVVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDIATQLVLCSEDDRLPVIQLWDLRFASSPLKVLESHSRGILSVSWSQADAELLLTSAKDSQILCRNLGSSEVVYKLPTQSSWCFDVQWCPRDPSVFSAASFNGWISLYAVMGRSWEVQHMRQADKV |
| 分子量 | 64.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structure of the Sec13-Sec31 complex and its role in COPII vesicle assembly"** by Fath, S., et al.
- **摘要**: 该研究解析了Sec13-Sec31(包括SEC31B)复合物的晶体结构，阐明其在COPII囊泡形成中的关键作用。作者通过重组人SEC31B蛋白与Sec13结合实验，揭示了复合物如何促进内质网膜的弯曲和囊泡出芽的分子机制。
2. **"Functional characterization of SEC31B in ER-Golgi transport and its implications in colorectal cancer"** by Liu, Y., et al.
- **摘要**: 研究利用重组人SEC31B蛋白进行体外囊泡形成实验，证实其与SEC23/SEC24复合物的互作对货物蛋白的包裹至关重要。此外，发现SEC31B在结直肠癌中表达异常，提示其潜在病理作用。
3. **"A SEC31B mutation disrupts COPII vesicle formation and causes neurodevelopmental disorders"** by Tan, X., et al.
- **摘要**: 通过重组SEC31B蛋白的功能分析，发现特定突变导致其与Sec13的结合能力下降，破坏COPII复合体的稳定性，从而引发神经发育障碍。研究为相关疾病的机制提供了实验依据。
4. **"Proteomic analysis of SEC31B interactions in the regulation of unconventional protein secretion"** by Chen, L., et al.
- **摘要**: 研究利用重组SEC31B蛋白进行免疫共沉淀结合质谱分析，鉴定出SEC31B与多种分泌途径相关蛋白的相互作用网络，揭示其在非经典蛋白分泌过程中的调控功能。
注：上述文献为示例，实际研究中建议通过PubMed或Web of Science检索具体论文。若需要真实文献，可尝试关键词“Recombinant human SEC31B COPII”或“SEC31B protein function”获取最新结果。
Secreted protein SEC31B (SEC31 homolog B, COPII coat complex component) is a key component of the COPII-coated vesicles involved in intracellular protein trafficking. As a paralog of SEC31A, it shares structural similarities, including WD40 repeat domains that facilitate vesicle coat assembly, but exhibits distinct tissue-specific expression patterns. SEC31B primarily functions in the endoplasmic reticulum (ER)-to-Golgi transport pathway by participating in the formation of COPII vesicles, which mediate cargo selection and vesicle budding.
This protein interacts with SEC13 to form the outer layer of the COPII coat, contributing to vesicle curvature and stabilization. Unlike SEC31A, which is ubiquitously expressed, SEC31B shows preferential expression in secretory cells and certain tissues like the pancreas and brain. Emerging studies suggest its potential role in maintaining ER homeostasis and regulating secretory capacity under stress conditions. Dysregulation of SEC31B has been implicated in pathological processes, including tumor progression and neurological disorders, though its specific mechanisms remain less characterized compared to SEC31A. Recent interest in SEC31B has grown due to its association with diabetes-related genes and potential involvement in specialized secretion pathways. Recombinant SEC31B protein serves as a critical tool for studying COPII complex dynamics, membrane trafficking defects, and disease-specific alterations in vesicular transport systems.
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