| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF214 |
| Uniprot No | Q9UL59 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-606 aa |
| 活性数据 | MAVTFEDVTI IFTWEEWKFL DSSQKRLYRE VMWENYTNVM SVENWNESYK SQEEKFRYLE YENFSYWQGW WNAGAQMYEN QNYGETVQGT DSKDLTQQDR SQCQEWLILS TQVPGYGNYE LTFESKSLRN LKYKNFMPWQ SLETKTTQDY GREIYMSGSH GFQGGRYRLG ISRKNLSMEK EQKLIVQHSY IPVEEALPQY VGVICQEDLL RDSMEEKYCG CNKCKGIYYW NSRCVFHKRN QPGENLCQCS ICKACFSQRS DLYRHPRNHI GKKLYGCDEV DGNFHQSSGV HFHQRVHIGE VPYSCNACGK SFSQISSLHN HQRVHTEEKF YKIECDKDLS RNSLLHIHQR LHIGEKPFKC NQCGKSFNRS SVLHVHQRVH TGEKPYKCDE CGKGFSQSSN LRIHQLVHTG EKSYKCEDCG KGFTQRSNLQ IHQRVHTGEK PYKCDDCGKD FSHSSDLRIH QRVHTGEKPY TCPECGKGFS KSSKLHTHQR VHTGEKPYKC EECGKGFSQR SHLLIHQRVH TGEKPYKCHD CGKGFSHSSN LHIHQRVHTG EKPYQCAKCG KGFSHSSALR IHQRVHAGEK PYKCREYYKG FDHNSHLHNN HRRGNL |
| 分子量 | 70.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF214蛋白的3-4篇参考文献示例，基于领域内常见研究方向归纳整理，供参考：
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1. **文献名称**：*ZNF214 suppresses cell proliferation and invasion via Wnt/β-catenin signaling in colorectal cancer*
**作者**：Liu Y, et al.
**摘要**：该研究探讨了ZNF214在结直肠癌中的功能，发现其作为肿瘤抑制因子通过抑制Wnt/β-catenin信号通路，降低癌细胞增殖和迁移能力。实验通过重组ZNF214蛋白过表达，证实其对下游靶基因的调控作用。
2. **文献名称**：*Structural insights into the DNA-binding mechanism of ZNF214 protein*
**作者**：Zhang L, et al.
**摘要**：本文解析了ZNF214蛋白的锌指结构域如何特异性结合DNA，利用重组蛋白进行体外结合实验和晶体结构分析，揭示了其在基因转录调控中的分子基础。
3. **文献名称**：*ZNF214 modulates epithelial-mesenchymal transition by interacting with SMAD3 in gastric cancer*
**作者**：Wang X, et al.
**摘要**：研究发现ZNF214在胃癌中通过重组蛋白表达干扰SMAD3信号通路，抑制上皮间质转化(EMT)，从而降低肿瘤转移潜能。体内外实验验证了其调控网络。
4. **文献名称**：*Proteomic analysis of ZNF214-associated complexes identifies novel cancer biomarkers*
**作者**：Chen H, et al.
**摘要**：通过质谱分析重组ZNF214蛋白相互作用组，鉴定出多个与癌症进展相关的蛋白伴侣，为ZNF214在肿瘤微环境中的功能提供机制线索。
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**注**：以上文献为示例性内容，实际研究可能需通过学术数据库(如PubMed、Web of Science)以“ZNF214 recombinant protein”或“ZNF214 function”为关键词检索获取具体信息。
Zinc finger protein 214 (ZNF214), a member of the Krüppel-associated box (KRAB) zinc finger protein family, is a transcription factor encoded by the *ZNF214* gene on human chromosome 11. Zinc finger proteins are characterized by tandem arrays of C2H2-type zinc finger motifs, which facilitate DNA or RNA binding and protein-protein interactions. While the precise biological role of ZNF214 remains partially understood, it is implicated in transcriptional regulation, potentially through chromatin remodeling or interaction with co-repressors. Studies suggest ZNF214 may function as a tumor suppressor, with reduced expression observed in cancers such as colorectal carcinoma and leukemia. Its dysregulation has been linked to abnormal cell proliferation, apoptosis, and metastasis. Epigenetic modifications, including promoter hypermethylation, are hypothesized to silence ZNF214 expression in malignancies. Recombinant human ZNF214 protein, produced via expression systems like *E. coli* or mammalian cells, enables functional studies to dissect its molecular mechanisms. This engineered protein retains DNA-binding capacity and is utilized in assays exploring its interaction with target genes, post-translational modifications, and regulatory pathways. Current research aims to clarify its role in cellular differentiation, oncogenesis, and potential therapeutic applications.
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