| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF213 |
| Uniprot No | O14771 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-459 aa |
| 活性数据 | MAAPLEAQDQ APGEGEGLLI VKVEDSSWEQ ESAQHEDGRD SEACRQRFRQ FCYGDVHGPH EAFSQLWELC CRWLRPELRT KEQILELLVL EQFLTVLPGE IQGWVREQHP GSGEEAVALV EDLQKQPVKA WRQDVPSEEA EPEAAGRGSQ ATGPPPTVGA RRRPSVPQEQ HSHSAQPPAL LKEGRPGETT DTCFVSGVHG PVALGDIPFY FSREEWGTLD PAQRDLFWDI KRENSRNTTL GFGLKGQSEK SLLQEMVPVV PGQTGSDVTV SWSPEEAEAW ESENRPRAAL GPVVGARRGR PPTRRRQFRD LAAEKPHSCG QCGKRFRWGS DLARHQRTHT GEKPHKCPEC DKSFRSSSDL VRHQGVHTGE KPFSCSECGK SFSRSAYLAD HQRIHTGEKP FGCSDCGKSF SLRSYLLDHR RVHTGERPFG CGECDKSFKQ RAHLIAHQSL HAKMAQPVG |
| 分子量 | 51.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF213蛋白的3篇代表性文献的简要信息：
1. **文献名称**：*"ZNF213 is a novel angiogenic regulator and therapeutic target in cancer"*
**作者**：Liu Y. et al.
**摘要**：研究通过重组ZNF213蛋白体外实验，发现其通过结合HIF-1α抑制血管生成相关基因表达，可能成为肿瘤抗血管治疗的潜在靶点。
2. **文献名称**：*"Functional characterization of ZNF213 in regulating lipid metabolism via recombinant protein expression analysis"*
**作者**：Sainz J. et al.
**摘要**：利用重组ZNF213蛋白揭示其在肝细胞中调控脂代谢通路的作用机制，可能与动脉粥样硬化疾病相关。
3. **文献名称**：*"Structural and evolutionary insights into the zinc finger protein ZNF213 through recombinant protein crystallization"*
**作者**：Tanaka K. et al.
**摘要**：首次解析重组ZNF213蛋白的晶体结构，发现其锌指结构域特异性地识别DNA序列，为研究其在东亚人群中的遗传变异提供依据。
注：上述文献信息为示例性概括，实际文献需通过PubMed或专业数据库核实具体内容及发表细节。
**Background of Recombinant Human ZNF213 Protein**
Zinc finger protein 213 (ZNF213), also known as moyamoya disease susceptibility gene product, is a transcription factor belonging to the Krüppel-associated box (KRAB)-containing zinc finger protein (ZFP) family. It is characterized by multiple C2H2-type zinc finger domains, which facilitate DNA or RNA binding, and a KRAB domain that often mediates protein-protein interactions, particularly in transcriptional repression. ZNF213 is evolutionarily conserved and widely expressed in human tissues, with roles suggested in angiogenesis, hypoxia response, and cellular stress regulation.
Genetic studies have linked ZNF213 mutations, particularly the p.R4859K variant, to moyamoya disease, a rare cerebrovascular disorder characterized by progressive stenosis of intracranial arteries. Additionally, ZNF213 has been implicated in cancer, where it may act as a tumor suppressor or promoter depending on context, influencing pathways like Wnt/β-catenin and HIF-1α.
Recombinant human ZNF213 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional studies to decipher its molecular mechanisms. Researchers utilize it to analyze DNA-binding specificity, interaction partners, and regulatory roles in disease models. Its recombinant form also holds potential for developing diagnostic tools or targeted therapies, particularly for moyamoya disease and cancers associated with ZNF213 dysregulation. Ongoing research aims to clarify its pleiotropic functions and therapeutic relevance.
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