| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF365 |
| Uniprot No | Q70YC4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-216 aa |
| 活性数据 | MSALGQITIT VSRCWNTERN QTDKNPCLHG AYLQLRETVK NKSTHLKKPL MKQAPPWKDH LTFQPLHPAE RKTQVWRWQS GNSSDLETTS SASPWPTGSN RDVVLNTLAE SCCGLSELIT APPYAGVSIQ GFSQIWVLFP FCGGTFHHNE KDVLGLQDFE RESVSTSQSR NISLLTLGQL QNCVIGKLTI IDLLTEHLLG VRHGVICFPW GLPSSS |
| 分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF365蛋白的研究文献示例(注：以下信息为模拟，实际文献需通过数据库检索验证)：
1. **文献名称**：*"ZNF365 regulates the transcription of DNA damage response genes and promotes genomic stability"*
**作者**：Smith A, et al.
**摘要**：研究揭示了ZNF365通过直接结合特定基因启动子区域调控DNA损伤修复通路(如ATM/ATR)相关基因的转录，重组ZNF365蛋白实验证明其增强细胞对电离辐射的抗性。
2. **文献名称**：*"Functional analysis of ZNF365 isoforms in cell cycle progression using recombinant protein models"*
**作者**：Wang X, et al.
**摘要**：利用重组ZNF365蛋白异构体进行体外实验，发现其不同剪切变体通过调控CDK抑制剂p21的表达影响G1/S期转换，提示在细胞周期调控中的异构体特异性功能。
3. **文献名称**：*"ZNF365 interacts with PARP1 to facilitate DNA repair: Insights from structural and biochemical assays"*
**作者**：Lee J, et al.
**摘要**：通过重组人ZNF365蛋白与PARP1的共纯化实验，证实二者在DNA单链断裂修复中形成复合物，晶体结构分析揭示了锌指结构域的关键结合位点。
4. **文献名称**：*"Association of ZNF365 polymorphisms and recombinant protein expression in inflammatory bowel disease"*
**作者**：Garcia R, et al.
**摘要**：基于重组ZNF365蛋白的功能研究，结合人群遗传学数据，发现特定SNP位点导致ZNF365表达降低，与肠道上皮屏障功能障碍相关。
建议通过PubMed或Web of Science搜索最新文献，关键词包括**"ZNF365 recombinant"**或**"ZNF365 protein function"**，以获取具体作者和发表信息。
**Background of Recombinant Human ZNF365 Protein**
The zinc finger protein 365 (ZNF365) belongs to the zinc finger protein family, characterized by conserved Cys₂/His₂ zinc finger domains that mediate sequence-specific DNA binding and protein-protein interactions. ZNF365 is encoded by the *ZNF365* gene, which has multiple splice variants. It plays critical roles in maintaining genomic stability, DNA damage repair, and cell cycle regulation. Studies suggest its involvement in the ATR kinase pathway, where it interacts with proteins like RPA1 to promote replication fork stability during DNA stress. Dysregulation of ZNF365 has been linked to cancer susceptibility, particularly breast cancer, and genetic variants in this gene are associated with autoimmune diseases such as Crohn’s disease.
Recombinant human ZNF365 protein is produced using genetic engineering techniques, typically expressed in *E. coli* or mammalian cell systems. This allows large-scale production of the protein for functional studies. Purification methods like affinity chromatography ensure high purity and biological activity. The recombinant protein is widely used to investigate ZNF365's molecular mechanisms in DNA repair, apoptosis, and cell proliferation. Structural studies employing techniques like X-ray crystallography or cryo-EM rely on recombinant ZNF365 to elucidate its interaction with DNA or partner proteins. Additionally, it serves as a tool for screening therapeutic compounds targeting pathways involving ZNF365. offering potential avenues for cancer therapy and disease biomarker development.
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