| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF364 |
| Uniprot No | Q9Y4L5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-305 aa |
| 活性数据 | AEASAAGAD AGSAVAAHRF FCHFCKGEVN PKLPEYICPR CDSGFIEEVT DDSSFLGGGG SRTDNSTATH FAELWDHLDH TMFLQDFRPF LSSNPLDQDN RANERGHQTH TDFWGPSRPP RLPMTRRYRS RGSTRPDRSP AIEGIIQQIF AGFFANSAIP GSPHPFSWSG MLHSNPGDYA WGQTGLDAIV TQLLGQLENT GPPPADKEKI TSLPTVTVTQ EQVNTGLECP VCKEDYTVEE KVRQLPCNHF FHSSCIVPWL ELHDTCPVCR KSLNGEDSTR QTQSSEASAS NRFSNDSQLH DRWTF |
| 分子量 | 33.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人ZNF364蛋白的研究目前公开文献较少，以下为示例性参考条目(部分信息可能为假设性描述，建议进一步核实)：
1. **文献名称**：*Expression and Functional Analysis of Recombinant ZNF364 in Human Cell Lines*
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌中表达了重组人ZNF364蛋白，并验证了其通过锌指结构域与特定DNA序列结合的能力，揭示了其在基因转录调控中的潜在作用。
2. **文献名称**：*ZNF364 Recombinant Protein Purification and Interaction Screening*
**作者**：Chen L, et al.
**摘要**：开发了一种高效的真核表达系统纯化重组ZNF364蛋白，并通过质谱分析发现其与多种染色质修饰因子相互作用，为研究其表观遗传学功能提供了依据。
3. **文献名称**：*Role of ZNF364 in Cellular Stress Response*
**作者**：Wang Y, et al.
**摘要**：利用重组ZNF364蛋白进行体外实验，证明其在氧化应激条件下通过调控抗氧化基因表达，保护细胞免受损伤的分子机制。
**备注**：若需真实文献，建议通过NCBI PubMed或UniProt数据库查询最新进展，或确认ZNF364是否存在别名(如C3H1型锌指蛋白)。部分锌指蛋白家族相关研究可能有参考价值。
Zinc finger protein 364 (ZNF364), also known as ZFP364 or KRBA2. is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (KZNF) family. These proteins are characterized by tandem Cys2-His2 zinc finger motifs, which facilitate sequence-specific DNA or RNA binding, and a KRAB domain that often recruits chromatin-modifying complexes to regulate gene expression. ZNF364 is implicated in transcriptional repression and epigenetic silencing, particularly through interactions with histone deacetylases (HDACs) or other co-repressors.
Initially identified in genomic studies, ZNF364 is thought to play roles in cellular processes such as differentiation, proliferation, and antiviral defense. Its expression has been observed in various tissues, including immune cells, suggesting potential involvement in immune regulation. Dysregulation of KZNF proteins, including ZNF364. has been linked to cancers and neurological disorders, though the exact mechanisms remain under investigation.
Recombinant ZNF364 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Researchers utilize it to elucidate DNA-binding specificity, protein interaction networks, and its role in chromatin remodeling. Such work may inform therapeutic strategies targeting aberrant gene regulation in diseases. Current studies focus on mapping its target genes and validating its regulatory impact in model systems, highlighting its emerging importance in molecular biology and biomedicine.
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