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Recombinant Human ZNF781 Protein

  • 中文名: 重组人(ZNF781)蛋白
  • 别    名: ZNF781Zinc finger Protein 781
货号: PAX2000-13006
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点ZNF781
Uniprot NoQ8N8C0
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-327 aa
活性数据MQRNAMYLKNVAETACNFQLTQYQISHANQKPYECQICGKPFRKRAHLTQHNRIHTGGKPYECKECGKVFICCSTLIQHKRTHTSEKPYECLECRKTFRRSAHLIRHQRIHTGEKPYKCKQCWKAFASVSDLIDIGKFTLMRDFTNVQNVGRHLTIAQLLFSIREFTLVRSPLNVRNVAKHSIIAQHLLNTRELILMRNLMNVRNVKRLLGKVHILLNIKEFILVRNHMSVSNVGRLSLVFLILIDIREFTLVKNPMNVKNVVELLTIVQLLFNTREFTLVRRLMNISSVGRFLSPVQHLFNIREHILMKNLMNVSNARRPSSIMHI
分子量64.7 kDa
蛋白标签GST-tag at N-terminal
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


参考文献

以下是关于重组人ZNF781蛋白的参考文献及简要摘要(注:ZNF781研究文献较少,以下为假设性示例,实际文献需根据数据库检索确认):

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1. **文献名称**:*ZNF781 functions as a transcriptional repressor and is frequently methylated in colorectal cancer*

**作者**:Chen L, et al.

**摘要**:本研究揭示了ZNF781在结直肠癌中作为转录抑制因子的功能,其启动子区域异常甲基化导致表达沉默。重组ZNF781蛋白通过结合靶基因启动子抑制肿瘤相关信号通路,提示其作为抑癌基因的潜在作用。

2. **文献名称**:*Recombinant expression and purification of human ZNF781 in E. coli: Structural insights into zinc finger domains*

**作者**:Smith K, et al.

**摘要**:报道了在大肠杆菌系统中高效表达并纯化重组ZNF781蛋白的方法,并通过质谱和圆二色谱分析其锌指结构域构象,为后续功能研究提供了蛋白工具。

3. **文献名称**:*Epigenetic silencing of ZNF781 promotes hepatocellular carcinoma progression by activating Wnt/β-catenin signaling*

**作者**:Wang Y, et al.

**摘要**:证实肝细胞癌中ZNF781因表观遗传修饰表达下调,重组ZNF781蛋白在体外实验抑制Wnt通路活性及癌细胞侵袭,表明其可能通过调控β-catenin发挥抗肿瘤功能。

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(注:以上文献为示例,实际引用需查询PubMed、Web of Science等平台获取真实数据。)


背景信息

ZNF781 is a member of the Krüppel-associated box (KRAB)-containing zinc finger protein (KZFP) family, characterized by tandem C2H2-type zinc finger domains that facilitate sequence-specific DNA binding. As a transcription regulator, it is implicated in epigenetic silencing through interactions with co-repressors like TRIM28/KAP1. recruiting histone-modifying complexes to mediate heterochromatin formation. Genomically, ZNF781 is located on chromosome 9q34.3. a region often associated with cancer-related epigenetic alterations. Functionally, ZNF781 has been studied in cellular differentiation, apoptosis, and tumor suppression.

Recombinant human ZNF781 protein, typically expressed in systems like *E. coli* or mammalian cells with purification tags (e.g., His-tag), enables biochemical studies to dissect its DNA-binding specificity, protein-protein interactions, and regulatory mechanisms. Studies suggest its dysregulation in cancers, including colorectal and cervical carcinomas, where promoter hypermethylation silences its expression, correlating with poor prognosis. Its tumor-suppressive role may involve repressing oncogenic pathways or viral defense, aligning with KZFP functions in genome stability and antiviral responses.

Despite progress, ZNF781's precise targets and pathways remain undercharacterized. Recombinant protein tools are vital for structural analyses (e.g., X-ray crystallography) and functional assays (e.g., luciferase reporter systems) to elucidate its role in disease, potentially unlocking therapeutic or diagnostic applications. Current research focuses on its interplay with chromatin modifiers and context-dependent roles in tumorigenesis.


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