| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP2B2 |
| Uniprot No | Q01814 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1243aa |
| 氨基酸序列 | MGDMTNSDFYSKNQRNESSHGGEFGCTMEELRSLMELRGTEAVVKIKETYGDTEAICRRLKTSPVEGLPGTAPDLEKRKQIFGQNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIISLGLSFYHPPGEGNEGCATAQGGAEDEGEAEAGWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVVRAGQVVQIPVAEIVVGDIAQVKYGDLLPADGLFIQGNDLKIDESSLTGESDQVRKSVDKDPMLLSGTHVMEGSGRMLVTAVGVNSQTGIIFTLLGAGGEEEEKKDKKGVKKGDGLQLPAADGAAASNAADSANASLVNGKMQDGNVDASQSKAKQQDGAAAMEMQPLKSAEGGDADDRKKASMHKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILVLYFTVDTFVVNKKPWLPECTPVYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQAYVGDVHYKEIPDPSSINTKTMELLINAIAINSAYTTKILPPEKEGALPRQVGNKTECGLLGFVLDLKQDYEPVRSQMPEEKLYKVYTFNSVRKSMSTVIKLPDESFRMYSKGASEIVLKKCCKILNGAGEPRVFRPRDRDEMVKKVIEPMACDGLRTICVAYRDFPSSPEPDWDNENDILNELTCICVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAIKCGIIHPGEDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTHTEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTETLLLRKPYGRNKPLISRTMMKNILGHAVYQLALIFTLLFVGEKMFQIDSGRNAPLHSPPSEHYTIIFNTFVMMQLFNEINARKIHGERNVFDGIFRNPIFCTIVLGTFAIQIVIVQFGGKPFSCSPLQLDQWMWCIFIGLGELVWGQVIATIPTSRLKFLKEAGRLTQKEEIPEEELNEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYEGLEKPESRTSIHNFMAHPEFRIEDSQPHIPLIDDTDLEEDAALKQNSSPPSSLNKNNSAIDSGINLTTDTSKSATSSSPGSPIHSLETSL |
| 预测分子量 | 136,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP2B2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: "Alternative splicing of the human plasma membrane calcium pump PMCA2 mRNA"
**作者**: Strehler, E.E., et al.
**摘要**: 该研究报道了ATP2B2(PMCA2)的多种剪接变体在哺乳动物细胞中的重组表达，分析了不同剪接形式对钙离子转运活性的影响，揭示了其组织特异性功能差异。
2. **文献名称**: "Crystal structure of the calcium pump with a bound ATP analogue"
**作者**: Toyoshima, C., et al.
**摘要**: 通过重组表达ATP2B2蛋白并解析其冷冻电镜结构，阐明了钙泵在ATP结合状态下的构象变化，为理解钙离子跨膜转运机制提供了结构基础。
3. **文献名称**: "A mutation in ATP2B2 causes hearing loss and vestibular dysfunction"
**作者**: Street, V.A., et al.
**摘要**: 研究利用重组ATP2B2蛋白进行功能验证，发现特定突变会破坏其钙转运能力，导致小鼠听力损失和前庭功能障碍，提示其在听觉系统中的关键作用。
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以上文献涵盖重组表达、结构解析及疾病机制研究，可为ATP2B2的功能和临床应用提供参考。
ATP2B2 recombinant protein is derived from the ATP2B2 gene, which encodes plasma membrane calcium-transporting ATPase 2 (PMCA2), a member of the P-type ATPase family. PMCA2 is a calcium efflux pump critical for maintaining cellular calcium homeostasis by actively transporting Ca²⁺ ions across the plasma membrane, thereby regulating intracellular calcium signaling. This protein is highly expressed in tissues sensitive to calcium fluctuations, such as cochlear hair cells, cardiac muscle, and neurons, where precise Ca²⁺ control is essential for physiological functions like hearing, muscle contraction, and neurotransmission.
Recombinant ATP2B2 is produced using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to generate purified, functional protein for research. Its production enables detailed studies of PMCA2’s structure, enzymatic activity, and interaction with regulatory partners, such as calmodulin, which modulates its calcium-pumping efficiency. Researchers utilize this recombinant protein to investigate PMCA2’s role in diseases linked to calcium dysregulation, including sensorineural hearing loss, neurodegenerative disorders (e.g., Alzheimer’s), and cardiovascular conditions. Mutations in ATP2B2 are associated with hereditary deafness in both humans and animal models, underscoring its importance in auditory physiology.
Additionally, ATP2B2 recombinant protein serves as a tool for drug discovery, aiding in screening compounds that modulate calcium pump activity. Its application extends to structural biology (e.g., cryo-EM studies) to resolve mechanistic details of calcium transport and ATP hydrolysis. By providing a controlled source of PMCA2. this recombinant protein advances understanding of calcium signaling pathologies and supports therapeutic development targeting calcium-related disorders.
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