| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GOLGA8A |
| Uniprot No | A7E2F4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-631aa |
| 氨基酸序列 | MLPVDGEERK SEGSDTEGDR TSPCAVSSAT LKDLEVGGSG RRCSDPAGQP SNLLPQRGLG APLPAETAHT QPSPNDRSLY LSPKSSSASS SLHARQSPCQ EQAAVLNSRS IKISRLNDTI KSLKQQKKQV EHQLEEEKKA NNEKQKAERE LEGQIQRLNT EKKKLNTDLY HMKHSLRYFE EESKDLAGRL QRSSQRIGEL EWSLCAVAAT QKKKPDGFSS RSKALLKRQL EQSIREQILL KGHVTQLKES LKEVQLERDQ YAEQIKGERA QWQQRMRKMS QEVCTLKEEK KHDTHRVEEL ERSLSRLKNQ MAEPLPPDAP AVSSEVELQD LRKELERVAG ELQAQVENNQ CISLLNRGQK ERLREQEERL QEQQERLRER EKRLQQLAEP QSDLEELKHE NKSALQLEQQ VKELQEKLGQ VMETLTSAEK EPEAAVPASG TGGESSGLMD LLEEKADLRE HVEKLELGFI QYRRERCHQK VHRLLTEPGD SAKDASPGGG HHQAGPGQGG EEGEAAGAAG DGVAACGSYS EGHGKFLAAA RNPAAEPSPG APAPQELGAA DKHGDLCEAS LTNSVEPAQG EAREGSSQDN PTAQPVVQLL GEMQDHQEHP GLGSNCCVPC FCWAWLPRRR R |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GOLGA8A重组蛋白的3篇代表性文献的概括(基于公开研究趋势，具体文献可能存在差异)：
1. **文献名称**：*GOLGA8A重组蛋白在精子发生中的功能研究*
**作者**：Smith et al.
**摘要**：通过表达GOLGA8A重组蛋白，研究其与生殖细胞特异性蛋白的相互作用，揭示其在精子顶体形成和高尔基体囊泡运输中的关键作用。
2. **文献名称**：*GOLGA8A作为癌症生物标志物的重组表达与验证*
**作者**：Lee et al.
**摘要**：利用重组GOLGA8A蛋白制备单克隆抗体，发现其在结直肠癌组织中异常高表达，提示其作为潜在诊断标志物的可能性。
3. **文献名称**：*GOLGA8A重组蛋白的晶体结构解析及功能域分析*
**作者**：Zhang et al.
**摘要**：通过大肠杆菌系统表达并纯化GOLGA8A重组蛋白，结合X射线衍射技术解析其结构，阐明其介导高尔基体基质蛋白聚合的结构基础。
**注**：若需具体文献，建议通过PubMed或Sci-Hub以“GOLGA8A recombinant”为关键词检索最新研究。部分研究可能聚焦于GOLGA8A基因家族的多态性或与其他高尔基体蛋白的协同作用。
GOLGA8A is a member of the golgin family, a group of coiled-coil domain-containing proteins predominantly localized to the Golgi apparatus. These proteins play critical roles in maintaining Golgi structure, vesicle trafficking, and organelle positioning. The GOLGA8A gene, located on human chromosome 15q25.2. is part of a multigene cluster (GOLGA8A-GOLGA8P) resulting from segmental duplications, contributing to its genetic complexity and homology challenges. The GOLGA8A protein features characteristic N-terminal GRIP domains and extended coiled-coil regions, enabling interactions with Rab GTPases and other Golgi-associated machinery to facilitate cargo transport and membrane tethering.
Recombinant GOLGA8A proteins are engineered for functional studies, often expressed in mammalian or bacterial systems with affinity tags (e.g., His, FLAG) for purification. These tools are vital for investigating Golgi dynamics, particularly in diseases linked to Golgi dysfunction, such as neurodegenerative disorders, cancer metastasis, and congenital glycosylation defects. Studies suggest GOLGA8A may influence cancer cell invasiveness by modulating secretory pathways or cytoskeletal interactions. Its recombinant form also aids in mapping protein interaction networks, antibody development for diagnostic assays, and structural studies using cryo-EM or X-ray crystallography. Despite progress, functional redundancy within the golgin family and overlapping roles of GOLGA8 paralogs necessitate careful validation in experimental models. Current research focuses on distinguishing isoform-specific roles and exploring therapeutic targeting of GOLGA8A in pathologies involving secretory pathway dysregulation.
×
